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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [4]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00051426-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00051426-M01, RRID:AB_547163
- Product name
- Mouse Monoclonal DNA Polymerase Kappa Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. POLK - polymerase (DNA directed) kappa
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Kynurenine Signaling Increases DNA Polymerase Kappa Expression and Promotes Genomic Instability in Glioblastoma Cells.
Both high-fidelity replicative and low-fidelity Y-family polymerases are involved in DNA rereplication.
Bostian AC, Maddukuri L, Reed MR, Savenka T, Hartman JH, Davis L, Pouncey DL, Miller GP, Eoff RL
Chemical research in toxicology 2016 Jan 19;29(1):101-8
Chemical research in toxicology 2016 Jan 19;29(1):101-8
Both high-fidelity replicative and low-fidelity Y-family polymerases are involved in DNA rereplication.
Sekimoto T, Oda T, Kurashima K, Hanaoka F, Yamashita T
Molecular and cellular biology 2015 Feb;35(4):699-715
Molecular and cellular biology 2015 Feb;35(4):699-715
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - analysis of DNA Polymerase Kappa in U87MG (lane 1), T98G (lane 2), A172 (lane 3) and normal human astrocyte (lane 4) lystes using anti-DNA Polymerase Kappa antibody. Each lane loaded with 10ug of samples. The primary antibody was used at a dilution of 1:5000 and incubated for 2 hours at room temperature in 0.1 % (w/v) BSA in PBS and 0.1% (v/v) tween-20. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Analysis of POLK over-expressed 293 cell line, cotransfected with POLK Validated Chimera RNAi ( Cat # H00051426-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with POLK monoclonal antibody (M01), clone 6F2 (Cat # H00051426-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Analysis of POLK expression in transfected 293T cell line by POLK monoclonal antibody (M01), clone 6F2.Lane 1: POLK transfected lysate(54.1 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - POLK monoclonal antibody (M01), clone 6F2 Analysis of POLK expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Detection limit for recombinant GST tagged POLK is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Analysis of monoclonal antibody to POLK on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Analysis of monoclonal antibody to POLK on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.
