PAB11246

antibody from Abnova Corporation

Targeting: POLK DINB1, DINP, POLQ

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB11246

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB11246, RRID:AB_1711489

Product name

POLK polyclonal antibody

Antibody type

Polyclonal

Description

Goat polyclonal antibody raised against synthetic peptide of POLK.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Translesion synthesis past 2'-deoxyxanthosine, a nitric oxide-derived DNA adduct, by mammalian DNA polymerases.

Yasui M, Suzuki N, Miller H, Matsuda T, Matsui S, Shibutani S
Journal of molecular biology 2004 Nov 26;344(3):665-74

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Crystal structure of the catalytic core of human DNA polymerase kappa.

Uljon SN, Johnson RE, Edwards TA, Prakash S, Prakash L, Aggarwal AK
Structure (London, England : 1993) 2004 Aug;12(8):1395-404

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A mechanism for the exclusion of low-fidelity human Y-family DNA polymerases from base excision repair.

Haracska L, Prakash L, Prakash S
Genes & development 2003 Nov 15;17(22):2777-85

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using POLK polyclonal antibody (Cat # PAB11246) shows detection ofa band ~98 KDa corresponding to human POLK (arrowhead).Approximately 35 ug of a HeLa whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.After blocking the membrane was probed withthe primary antibody diluted to 1 : 1,000.Reaction occurred overnight at 4°C followed bywashes and reaction with a 1 : 20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at roomtemperature.IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDyeis a trademark of LI-COR, Inc.