AM31124PU-N

antibody from Acris Antibodies GmbH

Targeting: POLK DINB1, DINP, POLQ

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM31124PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM31124PU-N, RRID:AB_10978821

Product name

anti DNA polymerase kappa

Antibody type

Monoclonal

Antigen

POLK antibody was raised against recombinant protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

6F2

Vial size

50 µg

Concentration

0,35 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western Blot analysis of POLK expression in HeLa nuclear extracts by POLK monoclonal antibody, clone 6F2.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western Blot analysis of POLK expression in transfected 293T cell line by POLK monoclonal antibody, clone 6F2.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis of POLK over-expressed 293 cell line, cotransfected with POLK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with POLK monoclonal antibody, clone 6F2. GAPDH (36.1 kDa) used as control.

ELISA

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged POLK is approximately 0.3ng/ml as a capture antibody.

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to POLK on HeLa cell. [antibody concentration 10 µg/ml]

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)