ABIN2508617

antibody from antibodies-online

Targeting: IL7 IL-7

Antibody data

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Validation data

Product number: ABIN2508617 - Provider product page
Provider: antibodies-online
Product name: anti-Interleukin 7 (IL7) antibody
Antibody type: Polyclonal
Antigen: Other
Description: Produced from sera of rabbits pre-immunized with highly pure recombinant Human IL-7. Anti-Human IL-7 specific antibody was purified by affinity chromatography employing immobilized Human IL-7 matrix.
Reactivity: Human
Host: Rabbit
Vial size: 100 μg
Storage: -20°C

IL7 protein structure - ABIN2508617 shown in red.

Supportive validation

Submitted by: antibodies-online (provider)
Main image
Experimental details: Western blot analysis of rat brain lysate: 1. Anti-mGluR1 (extracellular) antibody (ABIN2511221), (1:200). 2. Anti-mGluR1 (extracellular) antibody, preincubated with the control peptide antigen. Western blot analysis of mouse brain lysate: 1. Anti-mGluR1 (extracellular) antibody (ABIN2511221), (1:200). 2. Anti-mGluR1 (extracellular) antibody, preincubated with the control peptide antigen. Immunoprecipitation of rat brain lysate: 1. Cell lysate + protein A beads + Anti-mGluR1 (extracellular) antibody (ABIN2511221). 2. Cell lysate + protein A beads + pre-immune rabbit serum. 3. Cell lysate. Expression of mGluR1 in rat C6 glioma cells Immunocytochemical staining of live intact rat C6 glioma cells using Anti-mGluR1 (extracellular) antibody (ABIN2511221), (1:100), followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody (red). Nuclei were stained with Hoechst 33342 (blue). Indirect flow cytometry analysis in live intact Jurkat cells: Black: Unstained cells + goat-anti-rabbit-FITC. Green: Cells + Anti-mGluR1 (extracellular) antibody (ABIN2511221), (10 μg) + goat-anti-rabbit-FITC.

Supportive validation

Submitted by: antibodies-online (provider)
Main image
Experimental details: Western blot analysis of rat brain lysate: 1. Anti-mGluR1 (extracellular) antibody (ABIN2511221), (1:200). 2. Anti-mGluR1 (extracellular) antibody, preincubated with the control peptide antigen. Western blot analysis of mouse brain lysate: 1. Anti-mGluR1 (extracellular) antibody (ABIN2511221), (1:200). 2. Anti-mGluR1 (extracellular) antibody, preincubated with the control peptide antigen. Immunoprecipitation of rat brain lysate: 1. Cell lysate + protein A beads + Anti-mGluR1 (extracellular) antibody (ABIN2511221). 2. Cell lysate + protein A beads + pre-immune rabbit serum. 3. Cell lysate. Expression of mGluR1 in rat C6 glioma cells Immunocytochemical staining of live intact rat C6 glioma cells using Anti-mGluR1 (extracellular) antibody (ABIN2511221), (1:100), followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody (red). Nuclei were stained with Hoechst 33342 (blue). Indirect flow cytometry analysis in live intact Jurkat cells: Black: Unstained cells + goat-anti-rabbit-FITC. Green: Cells + Anti-mGluR1 (extracellular) antibody (ABIN2511221), (10 μg) + goat-anti-rabbit-FITC.

Supportive validation

Submitted by: antibodies-online (provider)
Main image
Experimental details: Western blot analysis of rat brain lysate: 1. Anti-mGluR1 (extracellular) antibody (ABIN2511221), (1:200). 2. Anti-mGluR1 (extracellular) antibody, preincubated with the control peptide antigen. Western blot analysis of mouse brain lysate: 1. Anti-mGluR1 (extracellular) antibody (ABIN2511221), (1:200). 2. Anti-mGluR1 (extracellular) antibody, preincubated with the control peptide antigen. Immunoprecipitation of rat brain lysate: 1. Cell lysate + protein A beads + Anti-mGluR1 (extracellular) antibody (ABIN2511221). 2. Cell lysate + protein A beads + pre-immune rabbit serum. 3. Cell lysate. Expression of mGluR1 in rat C6 glioma cells Immunocytochemical staining of live intact rat C6 glioma cells using Anti-mGluR1 (extracellular) antibody (ABIN2511221), (1:100), followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody (red). Nuclei were stained with Hoechst 33342 (blue). Indirect flow cytometry analysis in live intact Jurkat cells: Black: Unstained cells + goat-anti-rabbit-FITC. Green: Cells + Anti-mGluR1 (extracellular) antibody (ABIN2511221), (10 μg) + goat-anti-rabbit-FITC.