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LearnPA5-31076
antibody from Invitrogen Antibodies
Targeting: HEY1
bHLHb31, CHF-2, CHF2, HERP2, HESR-1, HESR1, HRT-1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
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- Product number
- PA5-31076 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HEY1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: DDDDK-tagged HEY1-transfected 293T. Predicted reactivity: Mouse (96%), Rat (89%), Xenopus laevis (81%), Dog (94%), Bovine (93%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.33 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HEY1 using 30 µg of A) A549 (B) H1299 (C) HCT116 and D) MCF-7 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a HEY1 polyclonal antibody (Product # PA5-31076) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of HEY1 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a HEY1 Polyclonal Antibody (Product # PA5-31076) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HEY1 showing staining in the nucleus and cytoplasm of H1299 cells. H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a HEY1 polyclonal antibody (Product # PA5-31076) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of HEY1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: HEY1 Polyclonal Antibody (Product # PA5-31076) diluted at 1:2000. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
