Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- A301-090A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CPSF73 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse CPSF73 by western blot (h&m). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 µg) and mouse NIH3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-CPSF73 antibody A301-090A used for WB at 0.1 µg/ml (A and B). CPSF73 was immunoprecipitated by rabbit anti-CPSF73 antibody A301-091A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CPSF73 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CPSF73 (Cat. No. A301-090A) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.