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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
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- Product number
- PA5-113198 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MRGPRX2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 3.353 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of MRGPRX2 in multiple sample types using a MRGPRX2 polyclonal antibody (Product # PA5-113198) at a concentration of 5 µg/ml. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:10,000. Sample types that were tested include: Lane 1: 293T whole cell lysate, Lane 2: Mouse liver tissue, Lane 3: MCF-7 whole cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MRGPRX2 in multiple sample types using a MRGPRX2 polyclonal antibody (Product # PA5-113198) at a dilution of 1:2,000. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:50,000. Sample types that were tested include: MCF-7 whole cell lysate, Hela whole cell lysate, and mouse liver tissue.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemichal analysis of MRGPRX2 in MCF-7 cells using a Polyclonal antibody (Product # PA5-113198) at a dilution of 1:100. An Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) secondary antibdoy was used.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of MRGPRX2 in MCF-7 cells. The sample was incubated with a MRGPRX2 polyclonal antibody (Product # PA5-113198) at a dilution of 1:200, followed by Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
