PAB29336

antibody from Abnova Corporation

Targeting: RPL30 L30

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29336

Provider

Abnova Corporation

Product name

RPL30 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human RPL30.

Antigen sequence

INSRLQLVMKSGKYVLGYKQTLKMIRQGKAKLVIL
ANNCPALRKSEIEYYAMLAKTGVHHYSGNNIELGT
ACGKYYRVCTLAIIDPGDSDIIRSMPEQTGEK


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Human cell line RT-4; Lane 2: Human cell line U-251MG sp with RPL30 polyclonal antibody (Cat# PAB29336) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with RPL30 polyclonal antibody (Cat# PAB29336) under 1-4 ug/mL working concentration shows positivity in cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebellum with RPL30 polyclonal antibody (Cat# PAB29336) shows cytoplasmic positivity in purkinje cells and cells of molecular layer at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)