PA5-89360

antibody from Invitrogen Antibodies

Targeting: RPL30 L30

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-89360

Provider

Invitrogen Antibodies

Product name

RPL30 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MVAAKKTKKS LESINSRLQL VMKSGKYVLG YKQTLKMIRQ GKAKLVILAN NCPALRKSEI EYYAMLAKTG VHHYSGNNIE LGTACGKYYR VCTLAIIDPG DSDIIRSMPE QTGEK; Positive Samples: HeLa, MCF7, HepG2, A-549, Mouse brain, Mouse heart, Mouse kidney, Rat liver

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.81 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of RPL30 was performed in HeLa cells using RPL30 Polyclonal Antibody (Product # PA5-89360).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Confocal immunofluorescence (Immunocytochemistry) analysis of RPL30 in HeLa cells. Samples were incubated with RPL30 Polyclonal antibody (Product # PA5-89360) using a dilution of 1:200. Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RPL30 in paraffin-embedded rat heart. Samples were incubated with RPL30 Polyclonal antibody (Product # PA5-89360) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RPL30 in paraffin-embedded human colon carcinoma. Samples were incubated with RPL30 Polyclonal antibody (Product # PA5-89360) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.