Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-29155 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CSRP3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa. Predicted reactivity: Mouse (97%), Rat (95%), Pig (98%), Chicken (87%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.8 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CSRP3 Polyclonal Antibody (Product # PA5-29155). Sample (30 µg of whole cell lysate). Lane A: Hela. 12% SDS PAGE. CSRP3 Polyclonal Antibody (Product # PA5-29155) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CSRP3 Polyclonal Antibody (Product # PA5-29155). Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with CSRP3 Polyclonal Antibody (Product # PA5-29155) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CSRP3 in paraformaldehyde-fixed HeLa cells using a CSRP3 polyclonal antibody (Product # PA5-29155) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CSRP3 Polyclonal Antibody detects CSRP3 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. CSRP3 stained by CSRP3 Polyclonal Antibody (Product # PA5-29155) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CSRP3 Polyclonal Antibody detects CSRP3 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. CSRP3 stained by CSRP3 Polyclonal Antibody (Product # PA5-29155) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CSRP3 Polyclonal Antibody detects CSRP3 protein at cytosol on C2C12 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded C2C12 xenograft. CSRP3 Polyclonal Antibody (Product # PA5-29155) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.