PA5-88674

antibody from Invitrogen Antibodies

Targeting: HIBADH NS5ATP1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-88674

Provider

Invitrogen Antibodies

Product name

HIBADH Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

6.79 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using HIBADH Polyclonal antibody (Product # PA5-88674) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 20s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of HIBADH in extracts of various cell lines using HIBADH Polyclonal Antibody (Product # PA5-88674) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of HIBADH in extracts of various cell lines using HIBADH Polyclonal Antibody (Product # PA5-88674) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of HIBADH was performed in C6 cells using HIBADH Polyclonal Antibody (Product # PA5-88674) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of HIBADH was performed in L929 cells using HIBADH Polyclonal Antibody (Product # PA5-88674) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of HIBADH was performed in U-2 OS cells using HIBADH Polyclonal Antibody (Product # PA5-88674) at a dilution of 1:100. Blue: DAPI for nuclear staining.