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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AF6716 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal ST8 alpha-2,8-Sialyltransferase 8A/ST8SIA1/Ganglioside GD3 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human ST8 alpha-2,8-Sialyltransferase 8A/ST8SIA1 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Sialidase inhibitors attenuate pulmonary fibrosis in a mouse model.
Karhadkar TR, Pilling D, Cox N, Gomer RH
Scientific reports 2017 Nov 8;7(1):15069
Scientific reports 2017 Nov 8;7(1):15069
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human ST8 alpha-2,8-Sialyltransferase 8A/ ST8SIA1 by Western Blot. Western blot shows lysates of Hs 294T human melanoma cell line, SK-Mel-28 human malignant melanoma cell line, A375 human melanoma cell line, and Bowes human melanoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human ST8 alpha-2,8-Sialyltransferase 8A/ST8SIA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6716) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ST8 alpha-2,8-Sialyltransferase 8A/ST8SIA1 at approximately 48-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ST8 alpha-2,8-Sialyltransferase 8A/ST8SIA1 in Human Breast Cancer Tissue. ST8 alpha-2,8-Sialyltransferase 8A/ST8SIA1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human ST8 alpha-2,8-Sialyltransferase 8A/ST8SIA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6716) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
