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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-51912 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPL3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: EDGKKQLEKD FSSMKKYCQV IRVIAHTQMR LLPLRQKKAH LMEIQVNGGT VAEKLDWARE RLEQQVPVNQ VFGQDEMIDV IGVTKGKGYK GVTSRWHTKK LPRKTHR Highest antigen sequence identity to the following orthologs: Mouse - 96%, Rat - 96%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RPL3 in Lane 1: Marker (kDa) 220, 112, 84, 47, 32, 26, 17; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a RPL3 Polyclonal Antibody (Product # PA5-51912).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of RPL3 in human cell line A-431 shows positivity in cytoplasm. Samples were probed using a RPL3 Polyclonal Antibody (Product # PA5-51912).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RPL3 in human cerebral cortex tissue shows strong cytoplasmic and nuclear (nucleolar) positivity in neuronal cells. Samples were probed using a RPL3 Polyclonal Antibody (Product # PA5-51912).
