66130-1-IG

antibody from Invitrogen Antibodies

Targeting: RPL3 L3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

66130-1-IG

Provider

Invitrogen Antibodies

Product name

RPL3 Monoclonal Antibody (2G1B8)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

2G1B8

Vial size

150 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RPL3 in various cell lines using Proteintech antibody 66130-1-IG at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 66130-1-Ig (RPL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RPL3 in various cell lines and tissues using Proteintech antibody 66130-1-IG at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 66130-1-Ig (RPL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 66130-1-Ig (RPL3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using (Product # 66130-1-IG) (RPL3 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells using 66130-1-IG (RPL3 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IGG.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human placenta slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 66130-1-IG ( RPL3 Antibody) at dilution of 1:600 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).