Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-30154 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPL3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, Jurkat, Raji. Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (93%), Xenopus laevis (94%), Dog (99%), Pig (99%), Chicken (97%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.74 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RPL3 Polyclonal Antibody (Product # PA5-30154). Sample (30 µg of whole cell lysate). Lane A: JurKat. Lane B: Raji. 10% SDS PAGE. RPL3 Polyclonal Antibody (Product # PA5-30154) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HeLa (Lane 2) and NIH/3T3 (Lane 3). The blot was probed with Anti-RPL3 Polyclonal Antibody (Product # PA5-30154, 1:5000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 49 kDa band corresponding to RPL3 was observed across the cell lines tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RPL3 in methanol-fixed A431 cells using a RPL3 polyclonal antibody (Product # PA5-30154) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of RPL3 was performed in paraffin-embedded mouse brain tissue using RPL3 Polyclonal Antibody (Product # PA5-30154) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of RPL3 was performed in paraffin-embedded mouse intestine tissue using RPL3 Polyclonal Antibody (Product # PA5-30154) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded 4T1 xenograft, using RPL3 (Product # PA5-30154) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.