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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- RQ6391 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PFAS Antibody / Phosphoribosylformylglycinamidine synthase
- Antibody type
- Polyclonal
- Description
- This highly specific PFAS antibody is suitable for use in Western blot/Immunofluorescence/Flow cytometry/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the PFAS antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) placenta, 2) A549, 3) HEK293, 4) HeLa, 5) K562, 6) U937, 7) HepG2 and rat 8) liver, 9) brain, 10) testis and mouse 11) liver, 12) brain and 13) testis tissue lysate with PFAS antibody. Predicted molecular weight ~145 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human HeLa cells with PFAS antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human HepG2 cells with PFAS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PFAS antibody.
