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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-30156 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPL9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa, HepG2, HCT116, mouse brain. Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (92%), Xenopus laevis (92%), Pig (100%), Chicken (95%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.8 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RPL9 Polyclonal Antibody (Product # PA5-30156). Sample (30 µg of whole cell lysate). Lane A: Hela. Lane B: Hep G2. Lane C: HCT116. 12% SDS PAGE. RPL9 Polyclonal Antibody (Product # PA5-30156) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RPL9 Polyclonal Antibody (Product # PA5-30156). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. RPL9 Polyclonal Antibody (Product # PA5-30156) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RPL9 in methanol-fixed HeLa cells using a RPL9 polyclonal antibody (Product # PA5-30156) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of RPL9 was performed in paraffin-embedded mouse eye tissue using RPL9 Polyclonal Antibody (Product # PA5-30156) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using RPL9 Polyclonal Antibody (Product # PA5-30156) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using RPL9 Polyclonal Antibody (Product # PA5-30156) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
