Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-85546 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDR2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution.
- Concentration
- 1 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CDR2 in A) HepG2 whole cell lysate using CDR2 polyclonal antibody (Product # PA5-85546) using 30 µg of sample at a dilution of 1:500. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CDR2 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a CDR2 Polyclonal Antibody (Product # PA5-85546) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CDR2 Polyclonal Antibody detects CDR2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDR2 protein stained by CDR2 Polyclonal Antibody (Product # PA5-85546) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CDR2 Polyclonal Antibody detects CDR2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDR2 protein stained by CDR2 Polyclonal Antibody (Product # PA5-85546) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CDR2 Polyclonal Antibody detects CDR2 protein on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. CDR2 Polyclonal Antibody (Product # PA5-85546) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of CDR2 in A) 293T cell lysate, B) control with pre-immune rabbit IgG, C) immunoprecipitation of CDR2 protein with CDR2 polyclonal antibody (Product # PA5-85546) using A) 30 µg, B) 2.5 µg, C) 2.5 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CDR2 Polyclonal Antibody immunoprecipitates CDR2 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 µg whole cell lysate/extract of CDR2 protein expressing 293T cells B : Control with 2.5 µg of pre-immune rabbit IgG C : Immunoprecipitation of CDR2 by 2.5 µg of CDR2 Polyclonal Antibody (Product # PA5-85546) 10% SDS-PAGE The immunoprecipitated CDR2 protein was detected by CDR2 Polyclonal Antibody (Product # PA5-85546) diluted at 1:1,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.
