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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-85546 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDR2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution. Predicted reactivity: Mouse (88%), Rat (90%), Rhesus Monkey (97%), Bovine (90%). Positive Control: U87-MG, SK-N-SH, IMR32, SK-N-AS. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CDR2 Polyclonal Antibody detects CDR2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDR2 protein stained by CDR2 Polyclonal Antibody (Product # PA5-85546) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CDR2 Polyclonal Antibody detects CDR2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDR2 protein stained by CDR2 Polyclonal Antibody (Product # PA5-85546) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CDR2 in paraffin-embedded breast cancer cells using CDR2 polyclonal antibody (Product # PA5-85546) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of CDR2 in A) 293T cell lysate, B) control with pre-immune rabbit IgG, C) immunoprecipitation of CDR2 protein with CDR2 polyclonal antibody (Product # PA5-85546) using A) 30 µg, B) 2.5 µg, C) 2.5 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CDR2 Polyclonal Antibody immunoprecipitates CDR2 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 µg whole cell lysate/extract of CDR2 protein expressing 293T cells B : Control with 2.5 µg of pre-immune rabbit IgG C : Immunoprecipitation of CDR2 by 2.5 µg of CDR2 Polyclonal Antibody (Product # PA5-85546) 10% SDS-PAGE The immunoprecipitated CDR2 protein was detected by CDR2 Polyclonal Antibody (Product # PA5-85546) diluted at 1:1,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.
