PA5-92978

antibody from Invitrogen Antibodies

Targeting: GDA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-92978

Provider

Invitrogen Antibodies

Product name

GDA Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: FVGKVCMDLN DTFPEYKETT EESIKETERF VSEMLQKNYS RVKPIVTPRF SLSCSETLMG ELGNIAKTRD LHIQSHISEN RDEVEAVKNL YPSYKNYTSV YDKNNLLTNK TVMAHGCYLS AEELNVFHER GASIAHCPNS NLSLSSGFLN VLEVLKHEVK IGLGTDVAGG YSYSMLDAIR RAVMVSNILL INKVNEKSLT LKEVFRLATL GGSQALGLDG EIGNFEVGKE FDAILINPKA SDSPIDLFYG DFFGDISEAV IQKFLYLGDD RNIEEVYVGG KQVVPFSSSV; Positive Samples: HeLa, SKOV3, mouse liver, mouse brain, rat brain, Mouse lung, Rat testis, NCI-H460

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.72 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using GDA Polyclonal antibody (Product # PA5-92978) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 30s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of GDA in extracts of various cell lines using GDA Polyclonal Antibody (Product # PA5-92978) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of A549 cells using GDA Polyclonal antibody (Product # PA5-92978). Blue: DAPI for nuclear staining.