Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [18]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 16490-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#16490-1-AP, RRID:AB_2146233
- Product name
- RPS12 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated RPS12 antibody (Cat. #16490-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Neurons with granulovacuolar degeneration bodies are resilient to tau-induced protein synthesis impairment.
Functional screen identifies RBM42 as a mediator of oncogenic mRNA translation specificity.
Ribosome customization and functional diversification among P-stalk proteins regulate late poxvirus protein synthesis.
RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages.
Functional screen for mediators of onco-mRNA translation specificity.
Repurposing colforsin daropate to treat MYC-driven high-grade serous ovarian carcinomas.
Axonal endoplasmic reticulum tubules control local translation via P180/RRBP1-mediated ribosome interactions.
Amelioration of gamma irradiation-induced salivary gland damage in mice using melatonin.
Haploinsufficiency of the essential gene Rps12 causes defects in erythropoiesis and hematopoietic stem cell maintenance.
RAPIDASH: A tag-free enrichment of ribosome-associated proteins reveals compositional dynamics in embryonic tissues and stimulated macrophages.
Identification of ribosomal protein L9 as a novel regulator of proinflammatory damage-associated molecular pattern molecules.
RNA G-quadruplex structures control ribosomal protein production.
HumanaFly: high-throughput transgenesis and expression of breast cancer transcripts in Drosophila eye discovers the RPS12-Wingless signaling axis.
On-Site Ribosome Remodeling by Locally Synthesized Ribosomal Proteins in Axons.
Downregulation of ribosome biogenesis during early forebrain development.
Progressive Differentiation and Instructive Capacities of Amniotic Fluid and Cerebrospinal Fluid Proteomes following Neural Tube Closure.
RPS12-specific shRNA inhibits the proliferation, migration of BGC823 gastric cancer cells with S100A4 as a downstream effector.
Recombineering-based dissection of flanking and paralogous Hox gene functions in mouse reproductive tracts.
Smits JFM, Ligthart TW, Jorge-Oliva M, Middelhoff S, Schipper F, Pita-Illobre D, Li KW, Scheper W
Science advances 2026 Mar 6;12(10):eaea8940
Science advances 2026 Mar 6;12(10):eaea8940
Functional screen identifies RBM42 as a mediator of oncogenic mRNA translation specificity.
Kovalski JR, Sarioglu G, Subramanyam V, Hernandez G, Rademaker G, Oses-Prieto JA, Slota M, Mohan N, Yiakis K, Liu I, Wen KW, Kim GE, Miglani S, Burlingame AL, Goodarzi H, Perera RM, Ruggero D
Nature cell biology 2025 Mar;27(3):518-529
Nature cell biology 2025 Mar;27(3):518-529
Ribosome customization and functional diversification among P-stalk proteins regulate late poxvirus protein synthesis.
Khalatyan N, Cornish D, Ferrell AJ, Savas JN, Shen PS, Hultquist JF, Walsh D
Cell reports 2025 Jan 28;44(1):115119
Cell reports 2025 Jan 28;44(1):115119
RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages.
Susanto TT, Hung V, Levine AG, Chen Y, Kerr CH, Yoo Y, Oses-Prieto JA, Fromm L, Zhang Z, Lantz TC, Fujii K, Wernig M, Burlingame AL, Ruggero D, Barna M
Molecular cell 2024 Sep 19;84(18):3545-3563.e25
Molecular cell 2024 Sep 19;84(18):3545-3563.e25
Functional screen for mediators of onco-mRNA translation specificity.
Kovalski JR, Sarioglu G, Subramanyam V, Hernandez G, Rademaker G, Oses-Prieto JA, Slota M, Mohan N, Yiakis K, Liu I, Wen KW, Kim GE, Miglani S, Burlingame AL, Goodarzi H, Perera RM, Ruggero D
bioRxiv : the preprint server for biology 2024 Oct 11;
bioRxiv : the preprint server for biology 2024 Oct 11;
Repurposing colforsin daropate to treat MYC-driven high-grade serous ovarian carcinomas.
Knarr MJ, Moon J, Rawat P, DiFeo A, Hoon DSB, Drapkin R
Science signaling 2024 Nov 19;17(863):eado8303
Science signaling 2024 Nov 19;17(863):eado8303
Axonal endoplasmic reticulum tubules control local translation via P180/RRBP1-mediated ribosome interactions.
Koppers M, Özkan N, Nguyen HH, Jurriens D, McCaughey J, Nguyen DTM, Li CH, Stucchi R, Altelaar M, MacGillavry HD, Kapitein LC, Hoogenraad CC, Farías GG
Developmental cell 2024 Aug 19;59(16):2053-2068.e9
Developmental cell 2024 Aug 19;59(16):2053-2068.e9
Amelioration of gamma irradiation-induced salivary gland damage in mice using melatonin.
Ren J, Zhu T, Wang Z, Lu L, Fan S
Journal of pineal research 2023 Sep;75(2):e12897
Journal of pineal research 2023 Sep;75(2):e12897
Haploinsufficiency of the essential gene Rps12 causes defects in erythropoiesis and hematopoietic stem cell maintenance.
Folgado-Marco V, Ames K, Chuen J, Gritsman K, Baker NE
eLife 2023 Jun 5;12
eLife 2023 Jun 5;12
RAPIDASH: A tag-free enrichment of ribosome-associated proteins reveals compositional dynamics in embryonic tissues and stimulated macrophages.
Susanto TT, Hung V, Levine AG, Kerr CH, Yoo Y, Chen Y, Oses-Prieto JA, Fromm L, Fujii K, Wernig M, Burlingame AL, Ruggero D, Barna M
bioRxiv : the preprint server for biology 2023 Dec 7;
bioRxiv : the preprint server for biology 2023 Dec 7;
Identification of ribosomal protein L9 as a novel regulator of proinflammatory damage-associated molecular pattern molecules.
Watanabe M, Toyomura T, Wake H, Nishinaka T, Hatipoglu OF, Takahashi H, Nishibori M, Mori S
Molecular biology reports 2022 Apr;49(4):2831-2838
Molecular biology reports 2022 Apr;49(4):2831-2838
RNA G-quadruplex structures control ribosomal protein production.
Varshney D, Cuesta SM, Herdy B, Abdullah UB, Tannahill D, Balasubramanian S
Scientific reports 2021 Nov 23;11(1):22735
Scientific reports 2021 Nov 23;11(1):22735
HumanaFly: high-throughput transgenesis and expression of breast cancer transcripts in Drosophila eye discovers the RPS12-Wingless signaling axis.
Katanaev VL, Kryuchkov M, Averkov V, Savitsky M, Nikolaeva K, Klimova N, Khaustov S, Solis GP
Scientific reports 2020 Dec 3;10(1):21013
Scientific reports 2020 Dec 3;10(1):21013
On-Site Ribosome Remodeling by Locally Synthesized Ribosomal Proteins in Axons.
Shigeoka T, Koppers M, Wong HH, Lin JQ, Cagnetta R, Dwivedy A, de Freitas Nascimento J, van Tartwijk FW, Ströhl F, Cioni JM, Schaeffer J, Carrington M, Kaminski CF, Jung H, Harris WA, Holt CE
Cell reports 2019 Dec 10;29(11):3605-3619.e10
Cell reports 2019 Dec 10;29(11):3605-3619.e10
Downregulation of ribosome biogenesis during early forebrain development.
Chau KF, Shannon ML, Fame RM, Fonseca E, Mullan H, Johnson MB, Sendamarai AK, Springel MW, Laurent B, Lehtinen MK
eLife 2018 May 10;7
eLife 2018 May 10;7
Progressive Differentiation and Instructive Capacities of Amniotic Fluid and Cerebrospinal Fluid Proteomes following Neural Tube Closure.
Chau KF, Springel MW, Broadbelt KG, Park HY, Topal S, Lun MP, Mullan H, Maynard T, Steen H, LaMantia AS, Lehtinen MK
Developmental cell 2015 Dec 21;35(6):789-802
Developmental cell 2015 Dec 21;35(6):789-802
RPS12-specific shRNA inhibits the proliferation, migration of BGC823 gastric cancer cells with S100A4 as a downstream effector.
Chen D, Zhang R, Shen W, Fu H, Liu S, Sun K, Sun X
International journal of oncology 2013 May;42(5):1763-9
International journal of oncology 2013 May;42(5):1763-9
Recombineering-based dissection of flanking and paralogous Hox gene functions in mouse reproductive tracts.
Raines AM, Adam M, Magella B, Meyer SE, Grimes HL, Dey SK, Potter SS
Development (Cambridge, England) 2013 Jul;140(14):2942-52
Development (Cambridge, England) 2013 Jul;140(14):2942-52
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 16490-1-AP(RPS12 antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using RPS12 antibody 16490-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human colon cancer using 16490-1-AP(RPS12 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human colon cancer using 16490-1-AP(RPS12 antibody) at dilution of 1:50 (under 40x lens)
- Sample type
- tissue
