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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- MA5-27697 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GABRD Monoclonal Antibody (N151/3)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibody was formerly sold as clone S151-3. 2 µg/mL of MA5-27697 was sufficient for detection of Delta1 GABA-A receptor in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 55kDa.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- N151/3
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GABRD in rat cell line lysates with 15 µg of sample. The sample was blocked with 1.5% BSA, incubated with GABRD monoclonal antibody (Product # MA5-27697) using a dilution of 1:1000 (2 hours at 4°C), followed by Sheep Anti-Mouse HRP (1 hour at RT).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of GABRD in human HaCaT cells. Sample was fixed with 100% cold methanol (10 minutes at -20°C), incubated with GABRD monoclonal antibody (Product # MA5-27697) using a dilution of 1:100 (1 hour at RT), and followed by FITC Goat Anti-Mouse (green) secondary antibody at a dilution of 1:50 (1 hour at RT).
