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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- NBP1-49979 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-49979, RRID:AB_10012169
- Product name
- Rabbit Polyclonal RPLP0 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Effects of sustained i.c.v. infusion of lupus CSF and autoantibodies on behavioral phenotype and neuronal calcium signaling.
Kapadia M, Bijelić D, Zhao H, Ma D, Stojanovich L, Milošević M, Andjus P, Šakić B
Acta neuropathologica communications 2017 Sep 7;5(1):70
Acta neuropathologica communications 2017 Sep 7;5(1):70
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: RPLP0 Antibody [NBP1-49979] - Detection of Human and Mouse RPLP0 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-RPLP0 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. RPLP0 was also immunoprecipitated by rabbit anti-RPLP0 antibody which recognizes an upstream epitope. For blotting immunoprecipitated RPLP0 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
