TA800481

antibody from OriGene

Targeting: ZFAND5 ZA20D2, ZFAND5A, ZNF216

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA800481

Provider

OriGene

Proper citation

OriGene Cat#TA800481, RRID:AB_2625484

Product name

ZFAND5 mouse monoclonal antibody, clone OTI5H8 (formerly 5H8)

Antibody type

Monoclonal

Description

ZFAND5 mouse monoclonal antibody, clone OTI5H8 (formerly 5H8)

Host

Mouse

Conjugate

Unconjugated

Epitope

ZFAND5

Isotype

IgG

Antibody clone number

OTI5H8

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZFAND5 (RC209943, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZFAND5.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-ZFAND5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ZFAND5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800481)

Validation comment

IHC