Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-32050 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CHMP4C Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- PA5-32050 targets CHMP4C in IHC (P) and WB applications and shows reactivity with Human and mouse samples. The PA5-32050 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 168 of Human CHMP4C. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CHMP4C using 20 µg of A) HeLa and B) HeLa cytoplasm lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a CHMP4C polyclonal antibody (Product # PA5-32050) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CHMP4C using 50 µg of mouse kidney lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a CHMP4C polyclonal antibody (Product # PA5-32050) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CHMP4C in paraffin-embedded U87 xenograft using a CHMP4C polyclonal antibody (Product # PA5-32050) at a dilution of 1:500.