Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX630413 - Provider product page
- Provider
- GeneTex
- Product name
- UQCRC1 antibody [GT139]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
No comments: Submit comment
Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with UQCRC1 antibody [GT139] (GTX630413) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- UQCRC1 antibody [GT139] detects UQCRC1 protein by western blot analysis.A. 50 £gg mouse brain lysate/extract7.5 % SDS-PAGEUQCRC1 antibody [GT139] (GTX630413) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- UQCRC1 antibody [GT139] detects UQCRC1 protein by western blot analysis.A. 50 £gg rat brain lysate/extract10 % SDS-PAGEUQCRC1 antibody [GT139] (GTX630413) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- UQCRC1 antibody [GT139] detects UQCRC1 protein by western blot analysis.A. 30 £gg 293T whole cell lysate/extractB. 30 £gg A431 whole cell lysate/extractC. 30 £gg HeLa whole cell lysate/extractD. 30 £gg HepG2 whole cell lysate/extract10 % SDS-PAGEUQCRC1 antibody [GT139] (GTX630413) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with UQCRC1 antibody [GT139] (GTX630413) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- UQCRC1 antibody [GT139] detects UQCRC1 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at room temperature.Green: UQCRC1 protein stained by UQCRC1 antibody [GT139] (GTX630413) diluted at 1:100.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
