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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
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- Product number
- GTX101896 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101896, RRID:AB_11178216
- Product name
- UQCRC1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with UQCRC1 antibody (GTX101896) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- UQCRC1 antibody detects UQCRC1 protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g A431 whole cell lysate/extract C. 30 ?g HeLa whole cell lysate/extractD. 30 ?g HepG2 whole cell lysate/extract E. 30 ?g A375 whole cell lysate/extract10% SDS-PAGEUQCRC1 antibody (GTX101896) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- UQCRC1 antibody detects UQCRC1 protein by western blot analysis.A Huh7 whole cell lysate/extractB Hep3B whole cell lysate/extractC HepG2 whole cell lysate/extract10% SDS-PAGEUQCRC1 antibody (GTX101896) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with UQCRC1 antibody (GTX101896) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NYREN18 antibody [N1N3] detects NYREN18 protein at mitochondria by immunofluorescent analysis.Sample: HepG2 cells were fixed in 2% paraformaldehyde/culture medium at 37¢J for 30 min.Green: NYREN18 protein stained by NYREN18 antibody [N1N3] (GTX101896) diluted at 1:500.Blue: Hoechst 33342 staining.
