Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 697602 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#697602, RRID:AB_2687187
- Product name
- Purified anti-UQCRC1
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- O91G6
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- HeLa cells were fixed with 4% paraformaldehyde (PFA) for fifteen minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 5% FBS for 60 minutes. Then the cells were stained with DAPI and intracellular anti-UQCRC1 antibody (clone O91G6 at 2 µg/ml) overnight at 4°C followed by Alexa Fluor® 594 (red) conjugated goat ant-mouse IgG for one hour at room temperature. The image was captured with a 60X objective.
- Conjugate
- Unconjugated