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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [6]
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- Product number
- PA5-97291 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATP5D Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MLPAALLRRP GLGRLVRHAR AYAEAAAAPA AASGPNQMSF TFASPTQVFF NGANVRQVDV PTLTGAFGIL AAHVPTLQVL RPGLVVVHAE DGTTSKYFVS SGSIAVNADS SVQLLAEEAV TLDMLDLGAA KANLEKAQAE LVGTADEATR AEIQIRIEAN EALVKALE; Positive Samples: MCF7, Raji, HepG2, A-549, Mouse kidney, Mouse heart, Mouse liver, Rat liver, Rat heart; Cellular Location: Mitochondrion, Mitochondrion inner membrane
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.64 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATP5D in extracts of various cell lines. Samples were incubated with ATP5D Polyclonal antibody (Product # PA5-97291) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ATP5D was performed in HeLa cells using ATP5D Polyclonal Antibody (Product # PA5-97291) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ATP5D was performed in C6 cells using ATP5D Polyclonal Antibody (Product # PA5-97291) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ATP5D was performed in HeLa cells using ATP5D Polyclonal Antibody (Product # PA5-97291) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of ATP5D in NIH/3T3 cells. Samples were incubated with ATP5D Polyclonal antibody (Product # PA5-97291) using a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of ATP5D in HeLa cells. Samples were incubated with ATP5D Polyclonal antibody (Product # PA5-97291) using a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of ATP5D in C6 cells. Samples were incubated with ATP5D Polyclonal antibody (Product # PA5-97291) using a dilution of 1:100. Blue: DAPI for nuclear staining.
