Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
- Other assay [1]
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Validation data
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- Product number
- PA5-117079 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SSBP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Samples: HL-60, MCF7, SW620
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.52 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SSBP1 using SSBP1 Polyclonal Antibody (Product # PA5-117079) at a 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of SSBP1 in U2OS cells using SSBP1 Polyclonal Antibody (Product # PA5-117079) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of SSBP1 in U2OS cells using SSBP1 Polyclonal Antibody (Product # PA5-117079) at a dilution of 1:100. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of SSBP1 in human colon carcinoma tissue using SSBP1 Polyclonal Antibody (Product # PA5-117079) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of SSBP1 in human breast cancer tissue using SSBP1 Polyclonal Antibody (Product # PA5-117079) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of SSBP1 in mouse spinal cord tissue using SSBP1 Polyclonal Antibody (Product # PA5-117079) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of SSBP1 in mouse brain tissue using SSBP1 Polyclonal Antibody (Product # PA5-117079) at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of SSBP1 in 200 µg extracts of MCF7 cells using SSBP1 Polyclonal Antibody (Product # PA5-117079). Western blot was performed from the immunoprecipitate using the same antibody at a dilution of 1:100