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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- HPA002866 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA002866, RRID:AB_1080092
- Product name
- Anti-SSBP1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human SSBP1, Gene description: single-stranded DNA binding protein 1, mitochondrial, Alternative Gene Names: mtSSB, SSBP, Validated applications: ICC, IHC, WB, Uniprot ID: Q04837, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Mitofusin 1 and 2 regulation of mitochondrial DNA content is a critical determinant of glucose homeostasis.
Self‐assembly of multi‐component mitochondrial nucleoids via phase separation
Mitochondrial RNA granules are critically dependent on mtDNA replication factors Twinkle and mtSSB
Reactivation of Dihydroorotate Dehydrogenase-Driven Pyrimidine Biosynthesis Restores Tumor Growth of Respiration-Deficient Cancer Cells
Mitochondrial Function of CKS2 Oncoprotein Links Oxidative Phosphorylation with Cell Division in Chemoradioresistant Cervical Cancer
Mitochondrial fusion is required for regulation of mitochondrial DNA replication
Dominant mutations in mtDNA maintenance gene SSBP1 cause optic atrophy and foveopathy
The mitochondrial outer-membrane location of the EXD2 exonuclease contradicts its direct role in nuclear DNA repair
Base-excision repair deficiency alone or combined with increased oxidative stress does not increase mtDNA point mutations in mice
Impaired Mitochondrial Fatty Acid Synthesis Leads to Neurodegeneration in Mice
Whole Cell Formaldehyde Cross-Linking Simplifies Purification of Mitochondrial Nucleoids and Associated Proteins Involved in Mitochondrial Gene Expression
Identification of Rep-Associated Factors in Herpes Simplex Virus Type 1-Induced Adeno-Associated Virus Type 2 Replication Compartments
Sidarala V, Zhu J, Levi-D'Ancona E, Pearson GL, Reck EC, Walker EM, Kaufman BA, Soleimanpour SA
Nature communications 2022 Apr 29;13(1):2340
Nature communications 2022 Apr 29;13(1):2340
Self‐assembly of multi‐component mitochondrial nucleoids via phase separation
Feric M, Demarest T, Tian J, Croteau D, Bohr V, Misteli T
The EMBO Journal 2021;40(6)
The EMBO Journal 2021;40(6)
Mitochondrial RNA granules are critically dependent on mtDNA replication factors Twinkle and mtSSB
Hensen F, Potter A, van Esveld S, Tarrés-Solé A, Chakraborty A, Solà M, Spelbrink J
Nucleic Acids Research 2019;47(7):3680-3698
Nucleic Acids Research 2019;47(7):3680-3698
Reactivation of Dihydroorotate Dehydrogenase-Driven Pyrimidine Biosynthesis Restores Tumor Growth of Respiration-Deficient Cancer Cells
Bajzikova M, Kovarova J, Coelho A, Boukalova S, Oh S, Rohlenova K, Svec D, Hubackova S, Endaya B, Judasova K, Bezawork-Geleta A, Kluckova K, Chatre L, Zobalova R, Novakova A, Vanova K, Ezrova Z, Maghzal G, Magalhaes Novais S, Olsinova M, Krobova L, An Y, Davidova E, Nahacka Z, Sobol M, Cunha-Oliveira T, Sandoval-Acuña C, Strnad H, Zhang T, Huynh T, Serafim T, Hozak P, Sardao V, Koopman W, Ricchetti M, Oliveira P, Kolar F, Kubista M, Truksa J, Dvorakova-Hortova K, Pacak K, Gurlich R, Stocker R, Zhou Y, Berridge M, Park S, Dong L, Rohlena J, Neuzil J
Cell Metabolism 2019;29(2):399-416.e10
Cell Metabolism 2019;29(2):399-416.e10
Mitochondrial Function of CKS2 Oncoprotein Links Oxidative Phosphorylation with Cell Division in Chemoradioresistant Cervical Cancer
Jonsson M, Fjeldbo C, Holm R, Stokke T, Kristensen G, Lyng H
Neoplasia 2019;21(4):353-362
Neoplasia 2019;21(4):353-362
Mitochondrial fusion is required for regulation of mitochondrial DNA replication
Barsh G, Silva Ramos E, Motori E, Brüser C, Kühl I, Yeroslaviz A, Ruzzenente B, Kauppila J, Busch J, Hultenby K, Habermann B, Jakobs S, Larsson N, Mourier A
PLOS Genetics 2019;15(6):e1008085
PLOS Genetics 2019;15(6):e1008085
Dominant mutations in mtDNA maintenance gene SSBP1 cause optic atrophy and foveopathy
Piro-Mégy C, Sarzi E, Tarrés-Solé A, Péquignot M, Hensen F, Quilès M, Manes G, Chakraborty A, Sénéchal A, Bocquet B, Cazevieille C, Roubertie A, Müller A, Charif M, Goudenège D, Lenaers G, Wilhelm H, Kellner U, Weisschuh N, Wissinger B, Zanlonghi X, Hamel C, Spelbrink J, Sola M, Delettre C
Journal of Clinical Investigation 2019;130(1):143-156
Journal of Clinical Investigation 2019;130(1):143-156
The mitochondrial outer-membrane location of the EXD2 exonuclease contradicts its direct role in nuclear DNA repair
Hensen F, Moretton A, van Esveld S, Farge G, Spelbrink J
Scientific Reports 2018;8(1)
Scientific Reports 2018;8(1)
Base-excision repair deficiency alone or combined with increased oxidative stress does not increase mtDNA point mutations in mice
Kauppila J, Bonekamp N, Mourier A, Isokallio M, Just A, Kauppila T, Stewart J, Larsson N
Nucleic Acids Research 2018;46(13):6642-6669
Nucleic Acids Research 2018;46(13):6642-6669
Impaired Mitochondrial Fatty Acid Synthesis Leads to Neurodegeneration in Mice
Nair R, Koivisto H, Jokivarsi K, Miinalainen I, Autio K, Manninen A, Poutiainen P, Tanila H, Hiltunen J, Kastaniotis A
The Journal of Neuroscience 2018;38(45):9781-9800
The Journal of Neuroscience 2018;38(45):9781-9800
Whole Cell Formaldehyde Cross-Linking Simplifies Purification of Mitochondrial Nucleoids and Associated Proteins Involved in Mitochondrial Gene Expression
Fujii H, Rajala N, Hensen F, Wessels H, Ives D, Gloerich J, Spelbrink J
PLOS ONE 2015;10(2):e0116726
PLOS ONE 2015;10(2):e0116726
Identification of Rep-Associated Factors in Herpes Simplex Virus Type 1-Induced Adeno-Associated Virus Type 2 Replication Compartments
Nicolas A, Alazard-Dany N, Biollay C, Arata L, Jolinon N, Kuhn L, Ferro M, Weller S, Epstein A, Salvetti A, Greco A
Journal of Virology 2010;84(17):8871-8887
Journal of Virology 2010;84(17):8871-8887
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SSBP1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human testis and pancreas tissues using HPA002866 antibody. Corresponding SSBP1 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
