Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF541 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Rat EphA5 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects rat EphA5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant mouse (rm) EphA4, rmEphA3, rmEphA6, rmEphA7, rmEphA8, and recombinant rat EphB1 is observed.
- Reactivity
- Human, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P54757- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- EphA5 in MCF-7 Human Cell Line. EphA5 was detected in immersion fixed MCF-7 human breast cancer cell line using Goat Anti-Human/Rat EphA5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF541) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to transmembrane proteins. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
