Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-13616 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDIA6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with mouse and rat based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references KDEL Receptor 1 Contributes to Cell Surface Association of Protein Disulfide Isomerases.
Bartels AK, Göttert S, Desel C, Schäfer M, Krossa S, Scheidig AJ, Grötzinger J, Lorenzen I
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2019;52(4):850-868
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2019;52(4):850-868
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a PDIA6 polyclonal antibody (Product # PA5-13616) in NCI-H460, MCF-7, A2058 cell lysates (35 µg per lane).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extract (30 µg lysate) of A549 (Lane 1), A549 treated with thapsigargin (2uM, 6 hrs) (Lane 2), SK-OV-3 (Lane 3), HCT116 (Lane 4), IM9 (Lane 5), HepG2 (Lane 6), and HeLa (Lane 7). The blot was probed with Anti-PDIA6 Polyclonal Antibody (Product # PA5-13616, 1:2000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 48 kDa band corresponding to PDIA6 was detected in cell lines tested and enhanced upon Thapsigargin treatment in A549 cell line.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human brain tissue using a PDIA6 polyclonal antibody (Product # PA5-13616), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of HeLa cells using a PDIA6 polyclonal antibody (Product # PA5-13616) (bottom), compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody