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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- PA5-31834 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDIA6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, Raji. Predicted reactivity: Mouse (94%), Rat (94%), Pig (94%), Rhesus Monkey (98%), Chimpanzee (99%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PDIA6 Polyclonal Antibody (Product # PA5-31834). Sample (30 µg of whole cell lysate). Lane A: Raji. 10% SDS PAGE. PDIA6 Polyclonal Antibody (Product # PA5-31834) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of PDIA6 was performed in paraffin-embedded mouse liver tissue using PDIA6 Polyclonal Antibody (Product # PA5-31834) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of PDIA6 was performed in paraffin-embedded rat liver tissue using PDIA6 Polyclonal Antibody (Product # PA5-31834) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of PDIA6 was performed in 293T whole cell extracts using 5 µg of PDIA6 Polyclonal Antibody (Product # PA5-31834). Samples were transferred to a membrane and probed with PDIA6 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
