Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- NB100-56485 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56485, RRID:AB_838069
- Product name
- Rabbit Polyclonal CRHR2/CRF2 Antibody
- Antibody type
- Polyclonal
- Description
- Protein G purified. There are three isoforms of CRHR2 in human: alpha is 411 amino acids, beta is 438 amino acids, and gamma is 397 amino acids.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Mast cell corticotropin-releasing factor subtype 2 suppresses mast cell degranulation and limits the severity of anaphylaxis and stress-induced intestinal permeability.
RGS1 expression is associated with poor prognosis in multiple myeloma.
Urocortin induces interleukin-6 release from rat cardiomyocytes through p38 MAP kinase, ERK and NF-kappaB activation.
D'Costa S, Ayyadurai S, Gibson AJ, Mackey E, Rajput M, Sommerville LJ, Wilson N, Li Y, Kubat E, Kumar A, Subramanian H, Bhargava A, Moeser AJ
The Journal of allergy and clinical immunology 2019 May;143(5):1865-1877.e4
The Journal of allergy and clinical immunology 2019 May;143(5):1865-1877.e4
RGS1 expression is associated with poor prognosis in multiple myeloma.
Roh J, Shin SJ, Lee AN, Yoon DH, Suh C, Park CJ, Huh J, Park CS
Journal of clinical pathology 2017 Mar;70(3):202-207
Journal of clinical pathology 2017 Mar;70(3):202-207
Urocortin induces interleukin-6 release from rat cardiomyocytes through p38 MAP kinase, ERK and NF-kappaB activation.
Huang M, Kempuraj D, Papadopoulou N, Kourelis T, Donelan J, Manola A, Theoharides TC
Journal of molecular endocrinology 2009 May;42(5):397-405
Journal of molecular endocrinology 2009 May;42(5):397-405
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: CRHR2/CRF2 Antibody [NB100-56485] - Shows a specific band for Corticotropin Releasing Factor 2 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CRHR2/CRF2 Antibody [NB100-56485] - Analysis of CRHR2 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Both of the isoforms detected by this antibody.