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- Antigen structure
- References [13]
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- Product number
- 459130 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NDUFS3 Monoclonal Antibody (3F9DD2)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Use Isolated mitochondria from Human, Bovine Rat and Mouse hearts; Cultured Human fibroblasts; HeLa cells; Human colon and cerebellum tissues as positive control.
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3F9DD2
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
Submitted references Involvement of the mitochondrial nuclease EndoG in the regulation of cell proliferation through the control of reactive oxygen species.
Low abundance of NDUFV2 and NDUFS4 subunits of the hydrophilic complex I domain and VDAC1 predicts mammalian longevity.
Herba Houttuyniae Extract Benefits Hyperlipidemic Mice via Activation of the AMPK/PGC-1α/Nrf2 Cascade.
Protection from α-Synuclein induced dopaminergic neurodegeneration by overexpression of the mitochondrial import receptor TOM20.
Cathepsin G Inhibition by Serpinb1 and Serpinb6 Prevents Programmed Necrosis in Neutrophils and Monocytes and Reduces GSDMD-Driven Inflammation.
L-Arabinose Elicits Gut-Derived Hydrogen Production and Ameliorates Metabolic Syndrome in C57BL/6J Mice on High-Fat-Diet.
Sixty years old is the breakpoint of human frontal cortex aging.
Enhanced GLUT4-Dependent Glucose Transport Relieves Nutrient Stress in Obese Mice Through Changes in Lipid and Amino Acid Metabolism.
Calsequestrins in skeletal and cardiac muscle from adult Danio rerio.
Cysteine dietary supplementation reverses the decrease in mitochondrial ROS production at complex I induced by methionine restriction.
Insulin and IGF-1 improve mitochondrial function in a PI-3K/Akt-dependent manner and reduce mitochondrial generation of reactive oxygen species in Huntington's disease knock-in striatal cells.
Independent and additive effects of atenolol and methionine restriction on lowering rat heart mitochondria oxidative stress.
IKKα and alternative NF-κB regulate PGC-1β to promote oxidative muscle metabolism.
Blasco N, Beà A, Barés G, Girón C, Navaridas R, Irazoki A, López-Lluch G, Zorzano A, Dolcet X, Llovera M, Sanchis D
Redox biology 2020 Oct;37:101736
Redox biology 2020 Oct;37:101736
Low abundance of NDUFV2 and NDUFS4 subunits of the hydrophilic complex I domain and VDAC1 predicts mammalian longevity.
Mota-Martorell N, Jove M, Pradas I, Sanchez I, Gómez J, Naudi A, Barja G, Pamplona R
Redox biology 2020 Jul;34:101539
Redox biology 2020 Jul;34:101539
Herba Houttuyniae Extract Benefits Hyperlipidemic Mice via Activation of the AMPK/PGC-1α/Nrf2 Cascade.
Cao K, Lv W, Liu X, Fan Y, Wang K, Feng Z, Liu J, Zang W, Xing L, Liu J
Nutrients 2020 Jan 7;12(1)
Nutrients 2020 Jan 7;12(1)
Protection from α-Synuclein induced dopaminergic neurodegeneration by overexpression of the mitochondrial import receptor TOM20.
De Miranda BR, Rocha EM, Castro SL, Greenamyre JT
NPJ Parkinson's disease 2020 Dec 8;6(1):38
NPJ Parkinson's disease 2020 Dec 8;6(1):38
Cathepsin G Inhibition by Serpinb1 and Serpinb6 Prevents Programmed Necrosis in Neutrophils and Monocytes and Reduces GSDMD-Driven Inflammation.
Burgener SS, Leborgne NGF, Snipas SJ, Salvesen GS, Bird PI, Benarafa C
Cell reports 2019 Jun 18;27(12):3646-3656.e5
Cell reports 2019 Jun 18;27(12):3646-3656.e5
L-Arabinose Elicits Gut-Derived Hydrogen Production and Ameliorates Metabolic Syndrome in C57BL/6J Mice on High-Fat-Diet.
Zhao L, Wang Y, Zhang G, Zhang T, Lou J, Liu J
Nutrients 2019 Dec 13;11(12)
Nutrients 2019 Dec 13;11(12)
Sixty years old is the breakpoint of human frontal cortex aging.
Cabré R, Naudí A, Dominguez-Gonzalez M, Ayala V, Jové M, Mota-Martorell N, Piñol-Ripoll G, Gil-Villar MP, Rué M, Portero-Otín M, Ferrer I, Pamplona R
Free radical biology & medicine 2017 Feb;103:14-22
Free radical biology & medicine 2017 Feb;103:14-22
Enhanced GLUT4-Dependent Glucose Transport Relieves Nutrient Stress in Obese Mice Through Changes in Lipid and Amino Acid Metabolism.
Gurley JM, Ilkayeva O, Jackson RM, Griesel BA, White P, Matsuzaki S, Qaisar R, Van Remmen H, Humphries KM, Newgard CB, Olson AL
Diabetes 2016 Dec;65(12):3585-3597
Diabetes 2016 Dec;65(12):3585-3597
Calsequestrins in skeletal and cardiac muscle from adult Danio rerio.
Furlan S, Mosole S, Murgia M, Nagaraj N, Argenton F, Volpe P, Nori A
Journal of muscle research and cell motility 2016 Apr;37(1-2):27-39
Journal of muscle research and cell motility 2016 Apr;37(1-2):27-39
Cysteine dietary supplementation reverses the decrease in mitochondrial ROS production at complex I induced by methionine restriction.
Gomez A, Gomez J, Lopez Torres M, Naudi A, Mota-Martorell N, Pamplona R, Barja G
Journal of bioenergetics and biomembranes 2015 Jun;47(3):199-208
Journal of bioenergetics and biomembranes 2015 Jun;47(3):199-208
Insulin and IGF-1 improve mitochondrial function in a PI-3K/Akt-dependent manner and reduce mitochondrial generation of reactive oxygen species in Huntington's disease knock-in striatal cells.
Ribeiro M, Rosenstock TR, Oliveira AM, Oliveira CR, Rego AC
Free radical biology & medicine 2014 Sep;74:129-44
Free radical biology & medicine 2014 Sep;74:129-44
Independent and additive effects of atenolol and methionine restriction on lowering rat heart mitochondria oxidative stress.
Sanchez-Roman I, Gomez A, Naudí A, Jove M, Gómez J, Lopez-Torres M, Pamplona R, Barja G
Journal of bioenergetics and biomembranes 2014 Jun;46(3):159-72
Journal of bioenergetics and biomembranes 2014 Jun;46(3):159-72
IKKα and alternative NF-κB regulate PGC-1β to promote oxidative muscle metabolism.
Bakkar N, Ladner K, Canan BD, Liyanarachchi S, Bal NC, Pant M, Periasamy M, Li Q, Janssen PM, Guttridge DC
The Journal of cell biology 2012 Feb 20;196(4):497-511
The Journal of cell biology 2012 Feb 20;196(4):497-511
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
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- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1. IKKalpha regulates mitochondrial biogenesis and fiber type specification in vivo. (A) Hind limbs from four independent litters of IKKalpha +/+ and IKKalpha -/- embryos (total n = 8) were isolated at E20. Cytochrome c and COX Va were measured by real-time quantitative RT-PCR. (B) Additional tissues from E20 limbs from A were homogenized and probed by Western blotting. (C) Total DNA was extracted from E20 limbs, and real-time RT-PCR analysis was performed to quantitate mitochondrial and nuclear (GAPDH) genome copy number. Results are means of five mice per genotype and calculated as fold mitochondrial over genomic DNA. P = 0.004. (D) AAV-GFP and AAV-IKKalpha viruses were injected into the TA. Mice were sacrificed after 3-4 mo, and protein lysates were prepared and analyzed by Western blotting. Shown is a representative blot from five independent experiments. (E and F) After similar viral injections as described in D, mitochondria were fractionated, and protein concentrations or DNA content was determined and normalized to total protein/DNA content (E; n = 5 per group; P < 0.005) or TA muscles were analyzed by EM (F). Bar, 1 um. Parallel muscles were stained for SDH. Bar, 200 um. Arrowheads point to individual mitochondria. (G) TA muscles were isolated from AAV-GFP- and AAV-IKKalpha-injected mice ( n = 3 per group, repeated five times), and myosin isoforms were measured by real-time RT-PCR. The data shown are from a single representative experiment out of three replica
