Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX113504 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113504, RRID:AB_11172615
- Product name
- NDUFS3 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Stable nuclear expression of ATP8 and ATP6 genes rescues a mtDNA Complex V null mutant.
Loss of mitochondrial DNA-encoded protein ND1 results in disruption of complex I biogenesis during early stages of assembly.
Boominathan A, Vanhoozer S, Basisty N, Powers K, Crampton AL, Wang X, Friedricks N, Schilling B, Brand MD, O'Connor MS
Nucleic acids research 2016 Nov 2;44(19):9342-9357
Nucleic acids research 2016 Nov 2;44(19):9342-9357
Loss of mitochondrial DNA-encoded protein ND1 results in disruption of complex I biogenesis during early stages of assembly.
Lim SC, Hroudová J, Van Bergen NJ, Lopez Sanchez MI, Trounce IA, McKenzie M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2016 Jun;30(6):2236-48
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2016 Jun;30(6):2236-48
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: THP-1 B: HL-60 12% SDS PAGE GTX113504 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NDUFS3 antibody detects NDUFS3 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody (GTX113504) diluted by 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and NDUFS3 knockout (KO) 293T cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody (GTX113504) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U87 xenograft, using NDUFS3(GTX113504) antibody at 1:500 dilution.
