RQ7236

antibody from NSJ Bioreagents

Targeting: NDUFS8 CI-23k, TYKY

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ7236

Provider

NSJ Bioreagents

Product name

NDUFS8 Antibody / NADH-ubiquinone oxidoreductase 23 kDa subunit

Antibody type

Polyclonal

Description

This highly specific NDUFS8 antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry/Direct ELISA applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the NDUFS8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat heart, 2) rat liver, 3) rat skeletal muscle, 4) mouse heart, 5) mouse liver, 6) mouse skeletal muscle and 7) mouse C2C12 cell lysate with NDUFS8 antibody. Expected molecular weight ~23 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HeLa, 2) HepG2, 3) A549, 4) MCF7, 5) Caco-2, 6) PC-3 and 7) 293T cell lysate with NDUFS8 antibody. Expected molecular weight ~23 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human liver cancer tissue with NDUFS8 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human liver cancer tissue with NDUFS8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE mouse heart tissue with NDUFS8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human 293T cells with NDUFS8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NDUFS8 antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human HeLa cells with NDUFS8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NDUFS8 antibody.