Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-B1675 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ PLK1 / PLK-1 Antibody (phospho-Thr210, clone 2A3) LS-B1675
- Antibody type
- Monoclonal
- Description
- Affinity purified
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2A3
- Storage
- Store at 4°C. Avoid freezing.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Panel A. Extracts from untreated Hela cells (Lane 1) or overnight nocodazole-treated Hela cells (Lane 2) were immunoprecipitated with the pan-PLK mAb (clone 3F8), resolved by electrophoresis, transferred to nitrocellulose and probed with mAb 2A3 reactive against Thr210-phosphorylated PLK-1. Proteins were visualized using an HRP goat anti-mouse secondary Ab and a chemiluminescence detection system. Panel B. Extracts from untreated Hela cells (Lane 1) or overnight nocodazole-treated Hela cells (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with mAb 2A3 reactive against Thr210-phosphorylated PLK-1. Proteins were visualized using an HRP goat anti-mouse secondary and a chemiluminescence detection system.