PA1-126

antibody from Invitrogen Antibodies

Targeting: PLK1 PLK

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-126

Provider

Invitrogen Antibodies

Product name

Anti-Phospho-PLK1 (Thr210) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recognizes human, mouse (weak), rat (weak) and Drosophila (weak). This antibody was originally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.

Reactivity

Human, Mouse, Rat, Drosophila

Host

Rabbit

Isotype

IgG

Vial size

200 µg

Concentration

0.6 mg/mL

Storage

-20°C

References

Submitted references

An Amino-Terminal Polo Kinase Interaction Motif Acts in the Regulation of Centrosome Formation and Reveals a Novel Function for centrosomin (cnn) in Drosophila.

Eisman RC, Phelps MA, Kaufman T
Genetics 2015 Oct;201(2):685-706

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Phospho-PLK1 pThr210 (green) in HeLa cells either left untreated (left panel) or treated with 50nM Nocodazole (right panel) for 16 hours. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a Phospho-PLK1 pThr210 polyclonal antibody (Product # PA1-126) at a dilution of 1:400 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan and ToxInsight Instrument at 20X magnification.