10305-1-AP

antibody from Proteintech Group

Targeting: PLK1 PLK

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

10305-1-AP

Provider

Proteintech Group

Product name

PLK1 antibody

Antibody type

Polyclonal

Description

KD/KO validated PLK1 antibody (Cat. #10305-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IHC, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

FBXW7 Targets the SPT6-ΔNp63 Axis for Degradation to Inhibit Esophageal Tumorigenesis Induced by 4-Nitroquinoline N-Oxide.

Zhang J, Yin L, You X, Xiong X, Sun Y
MedComm 2026 Mar;7(3):e70662

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BAG6 promotes hepatocellular carcinoma growth via enhancing PLK1-mediated aerobic glycolysis.

Zhang W, Du D, Lu H, Zhang W, Lei J
International journal of biological macromolecules 2026 Jan;338(Pt 1):149653

---

Activation of PBK by Gal-3 contributes to pulmonary artery hypertension by promoting PRC1 activation.

Zhang J, Liu J, Chen Y, Chen H, Meng Y, Wang Y, Qiu Y, Hu H, Shi W, Li M
Respiratory research 2026 Jan 29;27(1):65

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Methadone maintenance therapy alters the expression of PLK1 and IFIH1 in patients with heroin addiction: a case-control study.

Zhang B, Sang Y, Aihemaijiang M, Feng L, Muhedaner Y, Dong M, Hu X
Psychopharmacology 2026 Feb 27;

---

Novel driver gene FIRRM regulates the cell cycle for promoting tumor growth in hepatocellular carcinoma.

Ikehara T, Otsu H, Tobo T, Hosoda K, Hirose K, Ofuchi T, Tsujimoto A, Shibuta S, Kawata K, Itoyama S, Omachi K, Takahashi Y, Motomura Y, Hiraki Y, Ando Y, Hu Q, Yonemura Y, Shimizu A, Masuda T, Soejima Y, Mimori K
Journal of gastroenterology 2026 Apr 20;

---

PLK1 Mediates the Proliferation and Contraction of Airway Smooth Muscle Cells and Has a Role in T2-High Asthma with Neutrophilic Inflammation Model.

Pan Y, Xue Y, Fei X, Zhao L, Han L, Su H, Lin Y, Zhou Y, Zhang Y, Xie G, Kong D, Bao W, Zhang M
Journal of inflammation research 2025;18:4381-4394

---

Dehydrodiisoeugenol targets the PLK1-p53 axis to inhibit breast cancer cell cycle.

Li L, Zheng Y, Yang Y, Shi S, Liu S, Huang K, Qiu L, Zhang R, Huang W, Leng Y
Frontiers in pharmacology 2025;16:1545498

---

Comprehensive bioinformatics and in vitro studies reveal the carcinogenic role and molecular basis of endocrine disruptors in prostate cancer.

Yu F, Che B, Li W
Frontiers in cell and developmental biology 2025;13:1712195

---

Brusatol suppresses malignant progression of glioma via modulating the miR-574-5p/USP10/PLK1 axis.

Ma B, Wang L, Li J, Zhang B, Wang Y, Li N, Shen D, Han C
Phytomedicine : international journal of phytotherapy and phytopharmacology 2025 Oct;146:157142

---

ASNS Regulates H(2)O(2)-Induced Senescence, Oxidative Stress, and Glucose Metabolism in ARPE-19 Cells by Modulating USP13 Expression.

Xin X, Zhao X, Han X, Pu W
BioFactors (Oxford, England) 2025 Nov-Dec;51(6):e70057

---

Effect of PRKD3 on cell cycle in gastric cancer progression and downstream regulatory networks.

Wang S, Xie B, Deng H, Ma X, Tang B, Ma L, Zhu J, Li J, Li L
Medical oncology (Northwood, London, England) 2025 Mar 25;42(5):135

---

SHCBP1 is a novel regulator of PLK1 phosphorylation and promotes prostate cancer bone metastasis.

Tang C, Peng S, Chen Y, Cheng B, Li S, Zhou J, Wu Y, Li L, Zhong H, Guo Z, Lai Y, Huang H
MedComm 2025 Feb;6(2):e70082

---

Targeted biomimetic fusogenic liposome enhances tumor accumulation, penetration, and therapeutic efficacy of siRNA therapeutics.

Zhao X, Wu K, Zhang Y, Zhang H, Zhang X, Ni Q, Wang Z, Xia Y
Materials today. Bio 2025 Dec;35:102414

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SLX1 silencing overcomes Olaparib resistance in metastatic castration-resistant prostate cancer by disrupting SLX4-mediated DNA repair complexes.

Zhao X, Feng S, Nitie X, Muluo S, Lei Y
Cancer biology & therapy 2025 Dec;26(1):2545062

---

Polo-like kinase 1 drives hypoxia-induced renal fibrosis via PTEN/PGK1-mediated glycolytic activation.

Jiang A, Chen S, Yu X, Jia Y, Sun J, Bian Y, Du X, Gu X
International journal of biological macromolecules 2025 Aug;319(Pt 1):145305

---

A novel programmed cell death signature predicts clinical outcomes in clear cell renal cell carcinoma and identifies PLK1 as a therapeutic target.

Tan HT, Ma CY, Sun CH, Sun SZ, Zhang MX, Wang JF
Apoptosis : an international journal on programmed cell death 2025 Aug;30(7-8):1797-1825

---

Investigating Liquid-Liquid Phase Separation in Lung Adenocarcinoma to Improve Prognostic Accuracy and Treatment Efficacy.

Song Z, Li Y, Zhang P, Wei K, Zhu M, Wang Y, Li Z, Chen L, Zheng J
Journal of cellular and molecular medicine 2025 Aug;29(16):e70807

---

RASGEF1C affects aerobic glycolysis and facilitates lung cancer progression through the PLK1 signaling pathway.

Wu H, Zheng K, Han F
Expert review of anticancer therapy 2025 Aug;25(8):961-971

---

KIF2C promotes oral squamous cell carcinoma progression via PLK1 upregulation: implications for biomarker development and therapeutic targeting.

Wang C, Su Y, Shi J, Feng G
Journal of molecular histology 2025 Apr 28;56(3):146

---

Selective identification of epigenetic regulators at methylated genomic sites by SelectID.

Qian W, Jiang P, Niu M, Fu Y, Huang D, Zhang D, Liang Y, Wang Q, Han Y, Zeng X, Shi Y, Jiang L, Yu Z, Li J, Lu H, Wang H, Chen B, Qian P
Nature communications 2025 Apr 18;16(1):3709

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CYR61 Acts as an Intracellular Microtubule-Associated Protein and Coordinates Mitotic Progression via PLK1-FBW7 Pathway.

Hu K, Xie L, Wu W, Zhang J, Li Y, He J, Zhang Y, Lu D, Koeffler HP, Lin L, Yin D
International journal of biological sciences 2024;20(8):3140-3155

---

Biomimetic Nanosensitizer Potentiates Efficient Glioblastoma Gene-Radiotherapy through Synergistic Hypoxia Mitigation and PLK1 Silencing.

Chen J, Cui J, Jiao B, Zheng Z, Yu H, Wang H, Zhang G, Lai S, Gan Z, Yu Q
ACS applied materials & interfaces 2024 Oct 30;16(43):58241-58261

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USP18 promotes the proliferation, invasion, and migration of head and neck squamous cell carcinoma by deubiquitinating PLK1.

Geng L, Liu F, Yang L, Liu Y, Wu G
Experimental cell research 2024 Oct 1;442(2):114284

---

Targeted PLK1 suppression through RNA interference mediated by high-fidelity Cas13d mitigates osteosarcoma progression via TGF-β/Smad3 signalling.

Yuan Y, Cao D, Zhang A, Liu Z, Deng Z, Zhang S
Journal of cellular and molecular medicine 2024 May;28(10):e18400

---

Aurora-A condensation mediated by BuGZ aids its mitotic centrosome functions.

Zheng H, Zhang Q, Liu X, Shi F, Yang F, Xiang S, Jiang H
iScience 2024 May 17;27(5):109785

---

SHCBP1 Overexpression Aggravates Pancreatitis by Triggering the Loss of Primary Cilia.

Li L, Zhao H, Li Z, Shi W, Jiao Z
DNA and cell biology 2024 Mar;43(3):141-151

---

Apurinic/apyrimidinic endodeoxyribonuclease 1 (APE1) promotes stress granule formation via YBX1 phosphorylation in ovarian cancer.

Mao S, Xie C, Liu Y, Zhao Y, Li M, Gao H, Xiao Y, Zou Y, Zheng Z, Gao Y, Xie J, Tian B, Wang L, Hua Y, Xu H
Cellular and molecular life sciences : CMLS 2024 Mar 4;81(1):113

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CRISPR screens reveal convergent targeting strategies against evolutionarily distinct chemoresistance in cancer.

Zhong C, Jiang WJ, Yao Y, Li Z, Li Y, Wang S, Wang X, Zhu W, Wu S, Wang J, Fan S, Ma S, Liu Y, Zhang H, Zhao W, Zhao L, Feng Y, Li Z, Guo R, Yu L, Pei F, Hu J, Feng X, Yang Z, Yang Z, Yang X, Hou Y, Zhang D, Xu D, Sheng R, Li Y, Liu L, Wu HJ, Huang J, Fei T
Nature communications 2024 Jun 29;15(1):5502

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Tangeretin attenuates acute lung injury in septic mice by inhibiting ROS-mediated NLRP3 inflammasome activation via regulating PLK1/AMPK/DRP1 signaling axis.

Liu Y, Zhang Y, You G, Zheng D, He Z, Guo W, Antonina K, Shukhrat Z, Ding B, Zan J, Zhang Z
Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2024 Jan;73(1):47-63

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Aurora kinase A-mediated phosphorylation triggers structural alteration of Rab1A to enhance ER complexity during mitosis.

Zhang W, Zhang Z, Xiang Y, Gu DD, Chen J, Chen Y, Zhai S, Liu Y, Jiang T, Liu C, He B, Yan M, Wang Z, Xu J, Cao YL, Deng B, Zeng D, Lei J, Zhuo J, Lei X, Long Z, Jin B, Chen T, Li D, Shen Y, Hu J, Gao S, Liu Q
Nature structural & molecular biology 2024 Feb;31(2):219-231

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Proteomic analysis reveals that cigarette smoke exposure diminishes ovarian reserve in mice by disrupting the CREB1-mediated ovarian granulosa cell proliferation-apoptosis balance.

Xu M, Li F, Xu X, Hu N, Miao J, Zhao Y, Ji S, Wang Y, Wang L
Ecotoxicology and environmental safety 2024 Feb;271:115989

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Identification of crucial anoikis-related genes as novel biomarkers and potential therapeutic targets for lung adenocarcinoma via bioinformatic analysis and experimental verification.

Wu J, Zhang Y, You G, Guo W, Wang Y, Li J, Tan R, Fu X, Tang Y, Zan J, Su J
Aging 2024 Feb 9;16(3):2887-2907

---

STEAP4 with copper reductase activity suppresses tumorigenesis by regulating the cell cycle in hepatocellular carcinoma cells.

Yang T, Zou M, Xie Y, Zhang Y, Wang K, Jiang S, Zou Q
Cell division 2024 Dec 24;19(1):35

---

Reversal of the tamoxifen‑resistant breast cancer malignant phenotype by proliferation inhibition with bromosulfonamidine amino‑podophyllotoxin.

Wang J, Lv F, Zhu Y, Lu X, Zhang B
Oncology letters 2024 Aug;28(2):373

---

Phosphorylation of LZTS2 by PLK1 activates the Wnt pathway.

Liu R, Zhou D, Yu B, Zhou Z
Cellular signalling 2024 Aug;120:111226

---

Role of anoikis-related gene PLK1 in kidney renal papillary cell carcinoma: a bioinformatics analysis and preliminary verification on promoting proliferation and migration.

Gan L, Xiao Q, Zhou Y, Fu Y, Tang M
Frontiers in pharmacology 2023;14:1211675

---

Cascade dynamic assembly/disassembly of DNA nanoframework enabling the controlled delivery of CRISPR-Cas9 system.

Song N, Chu Y, Li S, Dong Y, Fan X, Tang J, Guo Y, Teng G, Yao C, Yang D
Science advances 2023 Sep;9(35):eadi3602

---

Tectoridin and PLK1 inhibitor synergistically promote the apoptosis of lung adenocarcinoma cells: Bioinformatic analysis of TCGA and TCMSP.

Wang M, Zhao F, Li Z, Li X, Dong L
Naunyn-Schmiedeberg's archives of pharmacology 2023 Oct;396(10):2417-2426

---

PLK1 inhibition dampens NLRP3 inflammasome-elicited response in inflammatory disease models.

Baldrighi M, Doreth C, Li Y, Zhao X, Warner E, Chenoweth H, Kishore K, Umrania Y, Minde DP, Thome S, Yu X, Lu Y, Knapton A, Harrison J, Clarke M, Latz E, de Cárcer G, Malumbres M, Ryffel B, Bryant C, Liu J, Lilley KS, Mallat Z, Li X
The Journal of clinical investigation 2023 Nov 1;133(21)

---

USP1 promotes the aerobic glycolysis and progression of T-cell acute lymphoblastic leukemia via PLK1/LDHA axis.

Liu S, Xiang Y, Wang B, Gao C, Chen Z, Xie S, Wu J, Liu Y, Zhao X, Yang C, Yue Z, Wang L, Wen X, Zhang R, Zhang F, Xu H, Zhai X, Zheng H, Zhang H, Qian M
Blood advances 2023 Jul 11;7(13):3099-3112

---

Parasympathetic-macrophages-ductal epithelial cells axis promotes female rat submandibular gland regeneration after excretory duct ligation/deligation.

Wang B, Li D, Li J, An W, Li Z, Shao Q, Qin L
Archives of oral biology 2023 Jan;145:105586

---

Comprehensive Analysis of Necroptosis Landscape in Skin Cutaneous Melanoma for Appealing its Implications in Prognosis Estimation and Microenvironment Status.

Cao X, He J, Chen A, Ran J, Li J, Chen D, Zhang H
Journal of personalized medicine 2023 Jan 29;13(2)

---

Targeting CBX3 with a Dual BET/PLK1 Inhibitor Enhances the Antitumor Efficacy of CDK4/6 Inhibitors in Prostate Cancer.

Liang H, Yang C, Zeng R, Song Y, Wang J, Xiong W, Yan B, Jin X
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023 Dec;10(36):e2302368

---

Increased degradation of FMRP contributes to neuronal hyperexcitability in tuberous sclerosis complex.

Winden KD, Pham TT, Teaney NA, Ruiz J, Chen R, Chen C, Sahin M
Cell reports 2023 Aug 29;42(8):112838

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Pan-Cancer Transcriptomic Analysis Identifies PLK1 Crucial for the Tumorigenesis of Clear Cell Renal Cell Carcinoma.

Qian Y, Li Y, Chen K, Liu N, Hong X, Wu D, Xu Z, Zhou L, Xu L, Jia R, Ge YZ
Journal of inflammation research 2022;15:1099-1116

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Vangl2 participates in the primary ciliary assembly under low fluid shear stress in hUVECs.

Sheng X, Gao S, Sheng Y, Xie X, Wang J, He Y
Cell and tissue research 2022 Jan;387(1):95-109

---

Oxcarbazepine induces mitotic catastrophe and apoptosis in NRK-52E proximal tubular cells.

Ota M, Funakoshi T, Aki T, Unuma K, Uemura K
Toxicology letters 2021 Oct 10;350:240-248

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Hyperactivation of HER2-SHCBP1-PLK1 axis promotes tumor cell mitosis and impairs trastuzumab sensitivity to gastric cancer.

Shi W, Zhang G, Ma Z, Li L, Liu M, Qin L, Yu Z, Zhao L, Liu Y, Zhang X, Qin J, Ye H, Jiang X, Zhou H, Sun H, Jiao Z
Nature communications 2021 May 14;12(1):2812

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Stimuli-Responsive and Highly Penetrable Nanoparticles as a Multifunctional Nanoplatform for Boosting Nonsmall Cell Lung Cancer siRNA Therapy.

Shi M, Wang Y, Zhao X, Zhang J, Hu H, Qiao M, Zhao X, Chen D
ACS biomaterials science & engineering 2021 Jul 12;7(7):3141-3155

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CDK1-PLK1/SGOL2/ANLN pathway mediating abnormal cell division in cell cycle may be a critical process in hepatocellular carcinoma.

Li L, Huang K, Zhao H, Chen B, Ye Q, Yue J
Cell cycle (Georgetown, Tex.) 2020 May;19(10):1236-1252

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PLK1 Inhibition alleviates transplant-associated obliterative bronchiolitis by suppressing myofibroblast differentiation.

Yu J, Xu H, Cui J, Chen S, Zhang H, Zou Y, Zhao J, Le S, Jiang L, Chen Z, Liu H, Zhang D, Xia J, Wu J
Aging 2020 Jun 15;12(12):11636-11652

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PHF7 is a novel histone H2A E3 ligase prior to histone-to-protamine exchange during spermiogenesis.

Wang X, Kang JY, Wei L, Yang X, Sun H, Yang S, Lu L, Yan M, Bai M, Chen Y, Long J, Li N, Li D, Huang J, Lei M, Shao Z, Yuan W, Zuo E, Lu K, Liu MF, Li J
Development (Cambridge, England) 2019 Jul 10;146(13)

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Translation control of the immune checkpoint in cancer and its therapeutic targeting.

Xu Y, Poggio M, Jin HY, Shi Z, Forester CM, Wang Y, Stumpf CR, Xue L, Devericks E, So L, Nguyen HG, Griselin A, Gordan JD, Umetsu SE, Reich SH, Worland ST, Asthana S, Barna M, Webster KR, Cunningham JT, Ruggero D
Nature medicine 2019 Feb;25(2):301-311

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pH-responsive hybrid nanoparticle with enhanced dissociation characteristic for siRNA delivery.

Shi M, Zhao X, Zhang J, Pan S, Yang C, Wei Y, Hu H, Qiao M, Chen D, Zhao X
International journal of nanomedicine 2018;13:6885-6902

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Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.

Song H, Guo T, Zhao Z, Wei Y, Luo H, Weng W, Zhang R, Zhong M, Chen C, Su J, Shen W
Biomaterials 2018 Sep;178:23-35

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Impact of iASPP on chemoresistance through PLK1 and autophagy in ovarian clear cell carcinoma.

Chan KK, Wong OG, Wong ES, Chan KK, Ip PP, Tse KY, Cheung AN
International journal of cancer 2018 Sep 15;143(6):1456-1469

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Effects of PLK1 on proliferation, invasion and metastasis of gastric cancer cells through epithelial-mesenchymal transition.

Song R, Hou G, Yang J, Yuan J, Wang C, Chai T, Liu Z
Oncology letters 2018 Nov;16(5):5739-5744

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53BP1 and BRCA1 control pathway choice for stalled replication restart.

Xu Y, Ning S, Wei Z, Xu R, Xu X, Xing M, Guo R, Xu D
eLife 2017 Nov 6;6

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

A375 cells were subjected to SDS PAGE followed by western blot with 10305-1-AP(PLK1 antibody) at dilution of 1:1000

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human cervical cancer using 10305-1-AP(PLK1 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The PLK1 antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human PLK1. This antibody recognizes human,mouse,rat antigen. The PLK1 antibody has been validated for the following applications: ELISA, IHC, WB analysis.

Appendix

10305-1-AP.pdf

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