AM33053PU-N

antibody from Acris Antibodies GmbH

Targeting: PLK1 PLK

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

AM33053PU-N

Provider

Acris Antibodies GmbH

Product name

anti PLK1

Antibody type

Monoclonal

Antigen

Amino Acids 300-603 of Human PLK-1

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

3F8

Vial size

0.1 mg

Concentration

0.5 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western Blot using PLK1 Antibody Cat.-No AM33053PU-N (Clone 3F8): Hela cell extract (Lane 1) or NIH3T3 cell extract (Lane 2) was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-Plk-1 (Clone 3F8) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunofluorescent microscope analysis of nocodazole treated Hela cells (10mM for overnight), using PLK1 Antibody Cat.-No AM33053PU-N (Clone 3F8) (green). Thr210-phosphorylated PLK-1 (poly6186) has been labeled with red and nuclei were stain with DAPI (blue).