37-7100
antibody from Invitrogen Antibodies
Targeting: PLK1
PLK
Antibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [9]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 37-7100 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PLK1 Monoclonal Antibody (36-298)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 36-298
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
Submitted references Feedback control of PLK1 by Apolo1 ensures accurate chromosome segregation.
Loss of aryl hydrocarbon receptor potentiates FoxM1 signaling to enhance self-renewal of colonic stem and progenitor cells.
O-GlcNAcylation of myosin phosphatase targeting subunit 1 (MYPT1) dictates timely disjunction of centrosomes.
Separase Inhibitor Sepin-1 Inhibits Foxm1 Expression and Breast Cancer Cell Growth.
Albatross/FBF1 contributes to both centriole duplication and centrosome separation.
A PIM-CHK1 signaling pathway regulates PLK1 phosphorylation and function during mitosis.
Insulin Signaling Regulates the FoxM1/PLK1/CENP-A Pathway to Promote Adaptive Pancreatic β Cell Proliferation.
Phosphorylation of PP1 Regulator Sds22 by PLK1 Ensures Accurate Chromosome Segregation.
Polo-like kinase 1 induces epithelial-to-mesenchymal transition and promotes epithelial cell motility by activating CRAF/ERK signaling.
FOXM1 and polo-like kinase 1 are co-ordinately overexpressed in patients with gastric adenocarcinomas.
Calmodulin activation of polo-like kinase 1 is required during mitotic entry.
Polo-like kinase 1 regulates cell proliferation and is targeted by miR-593* in esophageal cancer.
Disruptions of occludin and claudin-5 in brain endothelial cells in vitro and in brains of mice with acute liver failure.
Xu L, Ali M, Duan W, Yuan X, Garba F, Mullen M, Sun B, Poser I, Duan H, Lu J, Tian R, Ge Y, Chu L, Pan W, Wang D, Hyman A, Green H, Li L, Dou Z, Liu D, Liu X, Yao X
Cell reports 2021 Jul 13;36(2):109343
Cell reports 2021 Jul 13;36(2):109343
Loss of aryl hydrocarbon receptor potentiates FoxM1 signaling to enhance self-renewal of colonic stem and progenitor cells.
Han H, Davidson LA, Fan YY, Goldsby JS, Yoon G, Jin UH, Wright GA, Landrock KK, Weeks BR, Wright RC, Allred CD, Jayaraman A, Ivanov I, Roper J, Safe SH, Chapkin RS
The EMBO journal 2020 Oct 1;39(19):e104319
The EMBO journal 2020 Oct 1;39(19):e104319
O-GlcNAcylation of myosin phosphatase targeting subunit 1 (MYPT1) dictates timely disjunction of centrosomes.
Liu C, Shi Y, Li J, Liu X, Xiahou Z, Tan Z, Chen X, Li J
The Journal of biological chemistry 2020 May 22;295(21):7341-7349
The Journal of biological chemistry 2020 May 22;295(21):7341-7349
Separase Inhibitor Sepin-1 Inhibits Foxm1 Expression and Breast Cancer Cell Growth.
Zhang N, Pati D
Journal of cancer science & therapy 2018;10(3)
Journal of cancer science & therapy 2018;10(3)
Albatross/FBF1 contributes to both centriole duplication and centrosome separation.
Inoko A, Yano T, Miyamoto T, Matsuura S, Kiyono T, Goshima N, Inagaki M, Hayashi Y
Genes to cells : devoted to molecular & cellular mechanisms 2018 Dec;23(12):1023-1042
Genes to cells : devoted to molecular & cellular mechanisms 2018 Dec;23(12):1023-1042
A PIM-CHK1 signaling pathway regulates PLK1 phosphorylation and function during mitosis.
Adam K, Cartel M, Lambert M, David L, Yuan L, Besson A, Mayeux P, Manenti S, Didier C
Journal of cell science 2018 Aug 10;131(15)
Journal of cell science 2018 Aug 10;131(15)
Insulin Signaling Regulates the FoxM1/PLK1/CENP-A Pathway to Promote Adaptive Pancreatic β Cell Proliferation.
Shirakawa J, Fernandez M, Takatani T, El Ouaamari A, Jungtrakoon P, Okawa ER, Zhang W, Yi P, Doria A, Kulkarni RN
Cell metabolism 2017 Apr 4;25(4):868-882.e5
Cell metabolism 2017 Apr 4;25(4):868-882.e5
Phosphorylation of PP1 Regulator Sds22 by PLK1 Ensures Accurate Chromosome Segregation.
Duan H, Wang C, Wang M, Gao X, Yan M, Akram S, Peng W, Zou H, Wang D, Zhou J, Chu Y, Dou Z, Barrett G, Green HN, Wang F, Tian R, He P, Wang W, Liu X, Yao X
The Journal of biological chemistry 2016 Sep 30;291(40):21123-21136
The Journal of biological chemistry 2016 Sep 30;291(40):21123-21136
Polo-like kinase 1 induces epithelial-to-mesenchymal transition and promotes epithelial cell motility by activating CRAF/ERK signaling.
Wu J, Ivanov AI, Fisher PB, Fu Z
eLife 2016 Mar 22;5
eLife 2016 Mar 22;5
FOXM1 and polo-like kinase 1 are co-ordinately overexpressed in patients with gastric adenocarcinomas.
Dibb M, Han N, Choudhury J, Hayes S, Valentine H, West C, Sharrocks AD, Ang YS
BMC research notes 2015 Nov 14;8:676
BMC research notes 2015 Nov 14;8:676
Calmodulin activation of polo-like kinase 1 is required during mitotic entry.
Dai G, Qian Y, Chen J, Meng FL, Pan FY, Shen WG, Zhang SZ, Xue B, Li CJ
Biochemistry and cell biology = Biochimie et biologie cellulaire 2013 Oct;91(5):287-94
Biochemistry and cell biology = Biochimie et biologie cellulaire 2013 Oct;91(5):287-94
Polo-like kinase 1 regulates cell proliferation and is targeted by miR-593* in esophageal cancer.
Ito T, Sato F, Kan T, Cheng Y, David S, Agarwal R, Paun BC, Jin Z, Olaru AV, Hamilton JP, Selaru FM, Yang J, Matsumura N, Shimizu K, Abraham JM, Shimada Y, Mori Y, Meltzer SJ
International journal of cancer 2011 Nov 1;129(9):2134-46
International journal of cancer 2011 Nov 1;129(9):2134-46
Disruptions of occludin and claudin-5 in brain endothelial cells in vitro and in brains of mice with acute liver failure.
Chen F, Ohashi N, Li W, Eckman C, Nguyen JH
Hepatology (Baltimore, Md.) 2009 Dec;50(6):1914-23
Hepatology (Baltimore, Md.) 2009 Dec;50(6):1914-23
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of (A) recombinant Plk1 and (B) HeLa S3 cell lysates using Ms anti-Plk1 (clone 36-298, Product # 37-7100).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of T-47D (Lane 1), HCT 116 (Lane 2), K-562 (lane 3) and Raji (Lane 4). The blots were probed with Anti-PLK1 Mouse Monoclonal Antibody (Product # 37-7100, 1-3 µg/mL) and detected by chemiluminescence Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62-6520, 1:4000 dilution). A 66 kDa band corresponding to PLK 1was observed across cell lines tested, an extra band at ~38 kDa was observed in HCT 116 and K-562. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane using iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of (top row) HeLa S3 and (bottom row) NIH 3T3 cells using Ms anti-PLK-1 (Product # 37-7100). Image courtesy of Dr. Erich Nigg, Max-Planck Institut fur Biochemie, Germany.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of PLK-1 was done on 70% confluent log phase HCT 116 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PLK1 (36-298) Mouse Monoclonal Antibody (Product # 37-7100) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PLK1 showing weak cytoplasm and nucleus staining of paraffin-embedded human colon tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- PLK1 Monoclonal Antibody (36-298) (Product # 37-7100) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PLK1 showing staining in the cytoplasm and nucleus of paraffin-embedded mouse colon tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- PLK1 Monoclonal Antibody (36-298) (Product # 37-7100) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of PLK1 was done on K-562 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with PLK1 Mouse Monoclonal Antibody (377100, red histogram) or with mouse isotype control (yellow histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 2 The effects of PLK1 downregulation in EC cells. siPLK1 (siPLK286, siPLK785 and siPLK1412) or siNTC was transfected into EC cells. LF- and LF+ represent no transfection and transfection with Lipofectamine RNAiMAX alone, respectively. ( a ) Cell proliferation assay after PLK1-siRNA transfection. HSA/c, KYSE140, KYSE410 and OE33 cells were subjected to WST-8 assay 48 hr after the transfection. The absorbances at 450 nm were measured after 2-hr incubation with WST-8 reagent at 2, 4 and 6 days after plating cells. p values were calculated by two-way ANOVA. ( b ) Cell cycle analysis after PLK1-siRNA transfection. HSA/c and OE33 cells were stained with propidium iodide and analyzed by flow cytometry 48 hr after the siPLK1 transfection. Average percentages of cells in G2/M phase are shown in the representative histograms. ( c ) Localization of PLK1 in siRNA-treated HSA/c cells (magnification x200). HSA/c cells were stained with PLK1, which was labeled with AlexaFluor 568 (red), and nuclei were counterstained with 4',6-diamidine-2'-phenylindole dihydrochloride (DAPI, blue) 48 hr after the siRNA transfection. Left, cells stained with PLK1; middle, cells stained with DAPI; right, merged images. Top, cells transfected with siNTC; bottom, cells transfected with siPLK286. Photographs were taken for identical exposure intervals (70 msec for PLK1 and 3 msec for DAPI). PLK1 was localized mainly to the nuclei of mitotic cells, particularly at the spindle poles from prophase to metaphase (yel
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Assessment of AhR signaling in human colonic organoids A, B mRNA expression of human colonic stem cell markers LGR5 and OLFM4, n = 5 or 6 independent human samples. Paired Student's t -test. C Representative images of LGR5 ISH in human organoids treated with DMSO or TCDD. Scale bar 50 mum. D-G mRNA expression of target genes, n = 5 or 6 independent human samples. Paired Student's t -test. H Representative immunoblots for FoxM1 and PLK1 protein derived from organoids treated with DMSO or TCDD from n = 6 independent samples. Total protein was used as a loading control. I Determination of luciferase reporter activity of human FoxM1 in SW48 and Caco2 cell lines treated with DMSO or TCDD. n = 3-4 per group. J FOXM1 and CYP1A1 mRNA expression in different AhR WT and CRISPR KO clones treated with TCDD for 1 day. C56 and C61 designate different human subjects. K Validation of CRISPR KO clones was assessed by PCR amplification using primers flanking the targeted AhR exon. Subsequent sequencing revealed indels at the expected locations. L The association of FoxM1 and AhR mRNA expression in human normal colon biopsies ( n = 304). AhR low ( n = 152) is defined as below its median expression; AhR high ( n = 152) is defined as above its median expression. Dashed line indicates median, and dotted lines indicate 25 th /75 th quartiles. M Correlation between FoxM1 and Cyp1b1 mRNA expression in human normal colon biopsies ( n = 303) analyzed by Spearman rank correlation. N Representative brigh
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 4 TJ protein distribution in monolayers of bEnd3 cells assessed by immunofluorescent microscopy. The bEnd3 cells were transfected with vector alone (control column), with MMP-9 alone (MMP-9 column), or with both MMP-9 and TIMP-1 (MMP-9 + TIMP-1 column). TJ proteins were immunohistologically targeted for (A) occludin, (B) claudin-5, (C) ZO-1, and (D) ZO-2 (magnification x400). Bar = 50 mum.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 2 The effects of PLK1 downregulation in EC cells. siPLK1 (siPLK286, siPLK785 and siPLK1412) or siNTC was transfected into EC cells. LF- and LF+ represent no transfection and transfection with Lipofectamine RNAiMAX alone, respectively. ( a ) Cell proliferation assay after PLK1-siRNA transfection. HSA/c, KYSE140, KYSE410 and OE33 cells were subjected to WST-8 assay 48 hr after the transfection. The absorbances at 450 nm were measured after 2-hr incubation with WST-8 reagent at 2, 4 and 6 days after plating cells. p values were calculated by two-way ANOVA. ( b ) Cell cycle analysis after PLK1-siRNA transfection. HSA/c and OE33 cells were stained with propidium iodide and analyzed by flow cytometry 48 hr after the siPLK1 transfection. Average percentages of cells in G2/M phase are shown in the representative histograms. ( c ) Localization of PLK1 in siRNA-treated HSA/c cells (magnification x200). HSA/c cells were stained with PLK1, which was labeled with AlexaFluor 568 (red), and nuclei were counterstained with 4',6-diamidine-2'-phenylindole dihydrochloride (DAPI, blue) 48 hr after the siRNA transfection. Left, cells stained with PLK1; middle, cells stained with DAPI; right, merged images. Top, cells transfected with siNTC; bottom, cells transfected with siPLK286. Photographs were taken for identical exposure intervals (70 msec for PLK1 and 3 msec for DAPI). PLK1 was localized mainly to the nuclei of mitotic cells, particularly at the spindle poles from prophase to metaphase (yel