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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- TA328020 - Provider product page
- Provider
- OriGene
- Product name
- Mouse Monoclonal anti-PLK-1 Phospho (Thr210) Antibody
- Antibody type
- Monoclonal
- Description
- Mouse Monoclonal anti-PLK-1 Phospho (Thr210) Antibody
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PLK1
- Isotype
- IgG
- Antibody clone number
- 2A3
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Panel A. Extracts from untreated Hela cells (Lane 1) or overnight nocodazole-treated Hela cells (Lane 2) were immunoprecipitated with the pan-PLK mAb (clone 3F8), resolved by electrophoresis, transferred to nitrocellulose and probed with mAb 2A3 reactive against Thr210-phosphorylated PLK-1. Proteins were visualized using an HRP goat anti-mouse secondary Ab and a chemiluminescence detection system.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Panel B. Extracts from untreated Hela cells (Lane 1) or overnight nocodazole-treated Hela cells (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with mAb 2A3 reactive against Thr210-phosphorylated PLK-1. Proteins were visualized using an HRP goat anti-mouse secondary and a chemiluminescence detection system.
- Validation comment
- WB
