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LearnH00005347-M01
antibody from Novus Biologicals
Targeting: PLK1
PLK
Western blot ELISA Immunocytochemistry Immunoprecipitation
Immunohistochemistry Proximity ligation assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- H00005347-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005347-M01, RRID:AB_547120
- Product name
- Mouse Monoclonal PLK1 Antibody
- Antibody type
- Monoclonal
- Description
- Ascites. PLK1 - polo-like kinase 1 (Drosophila)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Liver kinase B1 regulates the centrosome via PLK1.
Werle K, Chen J, Xu HG, Zhao RX, He Q, Lu C, Cui R, Liang J, Li YL, Xu ZX
Cell death & disease 2014 Apr 10;5:e1157
Cell death & disease 2014 Apr 10;5:e1157
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of PLK1 expression in transfected 293T cell line by PLK1 monoclonal antibody (M01), clone 2G12.Lane 1: PLK1 transfected lysate(68.3 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of PLK1 in extracts from 293T, HeLa and A549 cell using anti-PLK1 monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PLK1 Antibody (2G12) [H00005347-M01] - PLK1 monoclonal antibody (M01), clone 2G12 Analysis of PLK1 expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: PLK1 Antibody (2G12) [H00005347-M01] - Detection limit for recombinant GST tagged PLK1 is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of monoclonal antibody to PLK1 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of PLK1 transfected lysate using anti-PLK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PLK1 MaxPab rabbit polyclonal antibody.
