ABIN117982

antibody from antibodies-online

Targeting: PLK1 PLK

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

ABIN117982

Provider

antibodies-online

Product name

anti-Polo-Like Kinase 1 (PLK1) (AA 205-214), (pThr210) antibody

Antibody type

Polyclonal

Antigen

Synthetic phospho peptide corresponding aa 205-214 of Human Polo-like kinase 1 (Plk1) protein

Description

Immunoaffinity Chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities

Reactivity

Human, Mouse, Rat, Canine, Chicken/Avian, Porcine, Xenopus, Zebrafish

Host

Rabbit

Antigen sequence

1 msaavtagkl arapadpgka gvpgvaapga
paaappakei pevlvdprsr rryvrgrflg 61
kggfakcfei sdadtkevfa gkivpkslll k
phqrekmsm eisihrslah qhvvgfhgff 121
edndfvfvvl elcrrrslle lhkrrkalte p
earyylrqi vlgcqylhrn rvihrdlklg 181
nlflnedlev kigdfglatk veydgerkkt l
cgtpnyiap evlskkghsf evdvwsigci 241
mytllvgkpp fetsclkety lrikkneysi p
khinpvaas liqkmlqtdp tarptinell 301
ndefftsgyi parlpitclt ipprfsiaps s
ldpsnrkpl tvlnkglenp lperprekee 361
pvvretgevv dchlsdmlqq lhsvnaskps e
rglvrqeea edpacipifw vskwvdysdk 421
yglgyqlcdn svgvlfndst rlilyndgds l
qyierdgte syltvsshpn slmkkitllk 481
yfrnymsehl lkaganitpr egdelarlpy l
rtwfrtrsa iilhlsngsv qinffqdhtk 541
lilcplmaav tyidekrdfr tyrlslleey g
cckelasrl ryartmvdkl lssrsasnrl 601
kas


Epitope

AA 205-214,pThr210

Vial size

0.1 mg

Concentration

1.0 mg/mL (by UV absorbance at 280 nm)

Storage

Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.

Handling

Avoid repeated freezing and thawing.

References

Submitted references

Polo-like kinase-1 controls recovery from a G2 DNA damage-induced arrest in mammalian cells.

van Vugt MA, Brás A, Medema RH
Molecular cell 2004 Sep 10;15(5):799-811

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Mitotic phosphorylation of the peripheral Golgi protein Nir2 by Cdk1 provides a docking mechanism for Plk1 and affects cytokinesis completion.

Litvak V, Argov R, Dahan N, Ramachandran S, Amarilio R, Shainskaya A, Lev S
Molecular cell 2004 May 7;14(3):319-30

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Polo-like kinase 1 (Plk1) inhibits p53 function by physical interaction and phosphorylation.

Ando K, Ozaki T, Yamamoto H, Furuya K, Hosoda M, Hayashi S, Fukuzawa M, Nakagawara A
The Journal of biological chemistry 2004 Jun 11;279(24):25549-61

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Hierarchical requirement of SWI/SNF in retinoblastoma tumor suppressor-mediated repression of Plk1.

Gunawardena RW, Siddiqui H, Solomon DA, Mayhew CN, Held J, Angus SP, Knudsen ES
The Journal of biological chemistry 2004 Jul 9;279(28):29278-85

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Polo-like kinase-1 is required for bipolar spindle formation but is dispensable for anaphase promoting complex/Cdc20 activation and initiation of cytokinesis.

van Vugt MA, van de Weerdt BC, Vader G, Janssen H, Calafat J, Klompmaker R, Wolthuis RM, Medema RH
The Journal of biological chemistry 2004 Aug 27;279(35):36841-54

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Polo-like kinase 1 is overexpressed in prostate cancer and linked to higher tumor grades.

Weichert W, Schmidt M, Gekeler V, Denkert C, Stephan C, Jung K, Loening S, Dietel M, Kristiansen G
The Prostate 2004 Aug 1;60(3):240-5