NBP1-48291

antibody from Novus Biologicals

Targeting: PLK1 PLK

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-48291

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-48291, RRID:AB_10010901

Product name

Mouse Monoclonal PLK1 Antibody

Antibody type

Monoclonal

Description

Affinity purified.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

2.5 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PLK1 Antibody (1D4) [NBP1-48291] Immunoprecipitation(IP) of PLK1 by using TrueMab monoclonal anti-PLK1 antibodies (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PLK1 Antibody (OTI1D4) [NBP1-48291] - (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PLK1 Antibody (OTI1D4) [NBP1-48291] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK1.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PLK1 Antibody (1D4) [NBP1-48291] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PLK1 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PLK1 Antibody (1D4) [NBP1-48291] - Staining of paraffin-embedded Human endometrium tissue using anti-PLK1 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PLK1 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PLK1 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PLK1 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Human endometrium tissue using anti-PLK1 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Human pancreas tissue using anti-PLK1 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Human prostate tissue using anti-PLK1 mouse monoclonal antibody.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: PLK1 Antibody (OTI1D4) [NBP1-48291] - HEK293T cells transfected with either PLK1 Human Tagged ORF Clone overexpress plasmid (Red), compared to an IgG isotype control, (Green) or empty vector control plasmid (Blue) were immunostained by anti-PLK1 antibody , and then analyzed by flow cytometry (1:100).