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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- NB100-61108 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-61108, RRID:AB_925571
- Product name
- Rabbit Polyclonal PLK1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specific for approx. 66k PLK phosphorylated at Thr210. Immunolabeling of the PLK band is completely blocked by lambda-phosphatase treatment.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: PLK1 [p Thr210] Antibody [NB100-61108] - Rat synaptic membrane showing specific immunolabeling of the ~66 k PLK protein phosphorylated at Thr210 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Thr210 PLK antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.
