Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- GTX104628 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX104628, RRID:AB_1241301
- Product name
- SDHB antibody [C2C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Mitochondrial proteomics with siRNA knockdown to reveal ACAT1 and MDH2 in the development of doxorubicin-resistant uterine cancer.
Lo YW, Lin ST, Chang SJ, Chan CH, Lyu KW, Chang JF, May EW, Lin DY, Chou HC, Chan HL
Journal of cellular and molecular medicine 2015 Apr;19(4):744-59
Journal of cellular and molecular medicine 2015 Apr;19(4):744-59
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Supportive validation
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- Experimental details
- SDHB antibody [C2C3], C-term detects SDHB protein by western blot analysis.A. 30 ?g THP-1 whole cell lysate/extractB. 30 ?g NCI-H929 whole cell lysate/extract12% SDS-PAGESDHB antibody [C2C3], C-term (GTX104628) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SDHB antibody [C2C3], C-term detects SDHB protein by western blot analysis.A. 30 ?g Neuro2A whole cell lysate/extract 12% SDS-PAGESDHB antibody [C2C3], C-term (GTX104628) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
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- Experimental details
- SDHB antibody [C2C3], C-term detects SDHB protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SDHB protein stained by SDHB antibody [C2C3], C-term (GTX104628) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
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- GeneTex (provider)
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- Experimental details
- SDHB antibody [C2C3], C-term detects SDHB protein at mitochondria on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. SDHB antibody [C2C3], C-term (GTX104628) diluted at 1:500.
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- Main image
- Experimental details
- SDHB antibody [C2C3], C-term detects SDHB protein at mitochindria on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. SDHB antibody [C2C3], C-term (GTX104628) diluted at 1:500.