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- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [3]
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- Product number
- PA5-23079 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SDHB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The target sequence has 92% sequence homology with rat, bovine and porcine, 85% sequence homology with xenopus and 84% sequence homology with zebrafish.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.14 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references SDHB and SDHA Immunohistochemistry in Canine Pheochromocytomas.
Abed FM, Brown MA, Al-Mahmood OA, Dark MJ
Animals : an open access journal from MDPI 2020 Sep 17;10(9)
Animals : an open access journal from MDPI 2020 Sep 17;10(9)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SDHB using a polyclonal antibody (Product # PA5-23079).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SDHB in mouse kidney lysate. Sample was incubated in SDHB polyclonal antibody (Product # PA5-23079).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of SDHB Polyclonal Antibody was achieved by transfecting HeLa with SDHB specific siRNAs (Silencer® select Product # s23229, s23230). Western blot analysis (Fig. a) was performed using whole cell extracts from the SDHB knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with SDHB Polyclonal Antibody (Product # PA5-23079, 1:7000 dilution) and Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to SDHB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-SDHB Polyclonal Antibody, (Product # PA5-23079) and a 30 kDa band corresponding to SDHB was observed across cell lines tested, with low level in MCF-7. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), Jurkat (Lane 2), MCF-7 (Lane 3), HEK-293 (Lane 4) and tissue extracts (40 µg lysate) of Mouse Kidney (Lane 5) and Rat Kidney (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with SDHB Polyclonal Antibody (Product # PA5-23079, 1:7000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SDHB using a polyclonal antibody (Product # PA5-23079).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of SDHB was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SDHB Polyclonal Antibody (Product # PA5-23079) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing mitochondrial localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of SDHB in mouse bladder. Samples were incubated in SDHB polyclonal antibody (Product # PA5-23079).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 ( A ) Succinate dehydrogenase A (SDHA) and ( B ) Succinate dehydrogenase B (SDHB) immunoreactivity in normal canine adrenal glands as controls.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Succinate dehydrogenase A (SDHA)/ Succinate dehydrogenase B (SDHB) immunoreactivity for all samples.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 SDHA and SDHB immunoreactivity. ( A , C , E )--SDHA immunohistochemistry. ( B , D , F )--SDHB immunoreactivity. ( A ) and ( B ) represent a case with both SDHA and SDHB immunoreactivity; ( C ) and ( D ) have SDHA but lack SDHB immunoreactivity; ( E ) and ( F ) represent a case lacking both SDHA and SDHB immunoreactivity. SDHA--Succinate dehydrogenase A, SDHB--Succinate dehydrogenase B, Original objective 40x.
