PA5-29843

antibody from Invitrogen Antibodies

Targeting: SDHB SDH, SDH1

Western blot

Antibody data

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Validation data

Product number: PA5-29843 - Provider product page
Provider: Invitrogen Antibodies
Product name: SDHB Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Recommended positive controls: NCI-H929, mouse brain. Predicted reactivity: Mouse (96%), Rat (96%), Zebrafish (90%), Xenopus laevis (93%), Dog (98%), Pig (98%), Chicken (90%), Rhesus Monkey (100%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 μL
Concentration: 0.42 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

SDHB protein structure - PA5-29843 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of SDHB showing staining in the mitochondria of HeLa cells. HeLa cells were fixed in ice-cold MeOH for 5 min and stained using a SDHB polyclonal antibody (Product # PA5-29843) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of SDHB was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SDHB Polyclonal Antibody (Product # PA5-29843) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Mitochondria (Panel c: red) was stained with Mitotracker Red CMXRos (Product # M7512). Panel d represents the merged image showing mitochondrial localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of SDHB was performed in HeLa cells fixed in MeOH for 5 min. Green: SDHB Polyclonal Antibody (Product # PA5-29843) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of SDHB was performed in HeLa cells fixed in MeOH for 5 min. Green: SDHB Polyclonal Antibody (Product # PA5-29843) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of SDHB was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SDHB Polyclonal Antibody (Product # PA5-29843) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Mitochondria (Panel c: red) was stained with Mitotracker Red CMXRos (Product # M7512). Panel d represents the merged image showing mitochondrial localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: SDHB Polyclonal Antibody detects SDHB protein at mitochondria on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. SDHB Polyclonal Antibody (Product # PA5-29843) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: SDHB Polyclonal Antibody detects SDHB protein at mitochondria on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. SDHB Polyclonal Antibody (Product # PA5-29843) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: SDHB Polyclonal Antibody detects SDHB protein at mitochondria on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. SDHB Polyclonal Antibody (Product # PA5-29843) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: SDHB Polyclonal Antibody detects SDHB protein at mitochondria on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. SDHB Polyclonal Antibody (Product # PA5-29843) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: SDHB Polyclonal Antibody detects SDHB protein at mitochondria on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. SDHB Polyclonal Antibody (Product # PA5-29843) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: SDHB Polyclonal Antibody detects SDHB protein at mitochondria on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. SDHB Polyclonal Antibody (Product # PA5-29843) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.