PA5-29843

antibody from Invitrogen Antibodies

Targeting: SDHB SDH, SDH1

Antibody data

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Validation data

SDHB protein structure - PA5-29843 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of SDHB using 30 µg NCI-H929 whole cell lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a SDHB polyclonal antibody (Product # PA5-29843) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using SDHB Polyclonal Antibody (Product # PA5-29843). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. SDHB Polyclonal Antibody (Product # PA5-29843) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using SDHB Polyclonal Antibody (Product # PA5-29843). Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with SDHB Polyclonal Antibody (Product # PA5-29843) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Liver (Lane 1), Rat Liver (Lane 2), Mouse Heart (Lane 3) and Mouse Adipose (Lane 4). The blot was probed with Anti-SDHB Polyclonal Antibody (Product # PA5-29843, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 32 kDa band corresponding to SDHB was observed across tissues tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of SDHB showing staining in the mitochondria of HeLa cells. HeLa cells were fixed in ice-cold MeOH for 5 min and stained using a SDHB polyclonal antibody (Product # PA5-29843) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of SDHB was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SDHB Polyclonal Antibody (Product # PA5-29843) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Mitochondria (Panel c: red) was stained with Mitotracker Red CMXRos (Product # M7512). Panel d represents the merged image showing mitochondrial localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of SDHB was performed in HeLa cells fixed in MeOH for 5 min. Green: SDHB Polyclonal Antibody (Product # PA5-29843) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: SDHB Polyclonal Antibody detects SDHB protein at mitochondria on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. SDHB Polyclonal Antibody (Product # PA5-29843) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: SDHB Polyclonal Antibody detects SDHB protein at mitochondria on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. SDHB Polyclonal Antibody (Product # PA5-29843) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: SDHB Polyclonal Antibody detects SDHB protein at mitochondria on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. SDHB Polyclonal Antibody (Product # PA5-29843) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: SDHB Polyclonal Antibody detects SDHB protein at mitochondria on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. SDHB Polyclonal Antibody (Product # PA5-29843) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: SDHB Polyclonal Antibody detects SDHB protein at mitochondria on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. SDHB Polyclonal Antibody (Product # PA5-29843) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: SDHB Polyclonal Antibody detects SDHB protein at mitochondria on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. SDHB Polyclonal Antibody (Product # PA5-29843) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.